| Improvement of the bitter taste of amino acids through the transpeptidation reaction of bacterial gamma-glutamyltranspeptidase. | |
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MedLine Citation:
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PMID: 11782200 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tastes of several bitter amino acids and their gamma-glutamyl derivatives were compared. The bitterness of Phe, Val, Leu, and His was reduced, sourness was produced, and preferences were increased by gamma-glutamylization. Because the effect of gamma-glutamylization of bitter amino acids was most obvious for Phe, which is an atypical bitter amino acid, an enzymatic method for the synthesis of gamma-glutamylphenylalanine (gamma-Glu-Phe) involving bacterial gamma-glutamyltranspeptidase was developed. The optimum reaction conditions were 200 mM Gln, 200 mM Phe, and 0.5 unit/mL GGT, pH 10.4. After 1.5-h of incubation at 37 degrees C, 140 mM gamma-Glu-Phe was obtained, the yield being 70%. gamma-Glu-Phe was purified on a Dowex 1x8 column and then identified by NMR. |
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Authors:
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Hideyuki Suzuki; Yoko Kajimoto; Hidehiko Kumagai |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 50 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2002-01-09 Completed Date: 2002-05-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 313-8 Citation Subset: IM |
Affiliation:
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Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. hideyuki@lif.kyoto-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis,
chemistry* Dipeptides / chemical synthesis, isolation & purification Escherichia coli / enzymology Magnetic Resonance Spectroscopy / methods Taste* gamma-Glutamyltransferase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Dipeptides; 7432-24-8/gamma-glutamylphenylalanine; EC 2.3.2.2/gamma-Glutamyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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