Document Detail

Improvement of arachidonic acid and eicosapentaenoic acid production by increasing the copy number of the genes encoding fatty acid desaturase and elongase into Pichia pastoris.
MedLine Citation:
PMID:  19306085     Owner:  NLM     Status:  MEDLINE    
Genes encoding Delta6 desaturase, Delta6 fatty acid elongase, and Delta5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Pichia pastoris to produce arachidonic acid (ARA; 20:4 Delta(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5 Delta(5, 8, 11, 14, 17)). A panel of Pichia clones carrying progressively increasing copies of the heterologous gene expression cassette was created using an in vitro multimerization approach. ARA and EPA accumulated up to 0.3 and 0.1% of total fatty acids, respectively, in the recombinant P. pastoris carrying with double copies of these three heterologous genes, as compared to 0.1 and 0.05%, respectively, in the recombinant P. pastoris with single copy.
Yun-Tao Li; Mao-Teng Li; Chu-Hua Fu; Peng-Peng Zhou; Jian-Min Liu; Long-Jiang Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-22
Journal Detail:
Title:  Biotechnology letters     Volume:  31     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-16     Completed Date:  2009-07-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1011-7     Citation Subset:  IM    
Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
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MeSH Terms
Acetyltransferases / genetics*,  metabolism
Arachidonic Acid / biosynthesis*
Diatoms / enzymology,  genetics
Eicosapentaenoic Acid / biosynthesis*
Fatty Acid Desaturases / genetics*,  metabolism
Gene Dosage*
Genes, Fungal
Pichia / enzymology*,  metabolism*
Recombinant Proteins / genetics,  metabolism
Reg. No./Substance:
0/Recombinant Proteins; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases

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