| Improvement of arachidonic acid and eicosapentaenoic acid production by increasing the copy number of the genes encoding fatty acid desaturase and elongase into Pichia pastoris. | |
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MedLine Citation:
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PMID: 19306085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genes encoding Delta6 desaturase, Delta6 fatty acid elongase, and Delta5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Pichia pastoris to produce arachidonic acid (ARA; 20:4 Delta(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5 Delta(5, 8, 11, 14, 17)). A panel of Pichia clones carrying progressively increasing copies of the heterologous gene expression cassette was created using an in vitro multimerization approach. ARA and EPA accumulated up to 0.3 and 0.1% of total fatty acids, respectively, in the recombinant P. pastoris carrying with double copies of these three heterologous genes, as compared to 0.1 and 0.05%, respectively, in the recombinant P. pastoris with single copy. |
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Authors:
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Yun-Tao Li; Mao-Teng Li; Chu-Hua Fu; Peng-Peng Zhou; Jian-Min Liu; Long-Jiang Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-22 |
Journal Detail:
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Title: Biotechnology letters Volume: 31 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-16 Completed Date: 2009-07-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1011-7 Citation Subset: IM |
Affiliation:
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Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases
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genetics*,
metabolism Arachidonic Acid / biosynthesis* Diatoms / enzymology, genetics Eicosapentaenoic Acid / biosynthesis* Fatty Acid Desaturases / genetics*, metabolism Gene Dosage* Genes, Fungal Pichia / enzymology*, metabolism* Recombinant Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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