| Improvement of L-lactic acid production by osmotic-tolerant mutant of Lactobacillus casei at high temperature. | |
| | |
MedLine Citation:
|
PMID: 20857288 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
L-Lactic acid production by Lactobacillus casei was used as a model to study the mechanism of substrate inhibition and the strategy for enhancing L-lactic acid production. It was found that the concentration of cell growth and L-lactate decreased with the increase of glucose concentration and fermentation temperature. To enhance the osmotic stress resistance of the strain at high temperature, a mutant G-03 was screened and selected with 360 g/L glucose at 45°C as the selective criterion. To further increase the cell growth for lactic acid production, 3 g/L of biotin was supplemented to the medium. As a result, L: -lactate concentration by the mutant G-03 reached 198.2 g/L (productivity of 5.5 g L(-1) h(-1)) at 41°C in a 7-L fermentor with 210 g/L glucose as carbon source. L: -Lactate concentration and productivity of mutant G-03 were 115.2% and 97.8% higher than those of the parent strain, respectively. The strategy for enhancing L: -lactic acid production by increasing osmotic stress resistance at high temperature may provide an alternative approach to enhance organic acid production with other strains. |
| | |
Authors:
|
Xiang-Yang Ge; Jian Yuan; Hao Qin; Wei-Guo Zhang |
Related Documents
:
|
16402248 - The effects of propionibacterium acidipropionici and lactobacillus plantarum, applied a... 22579008 - Intestinal ischemia-reperfusion increases efflux for uric acid via paracellular route i... 7747968 - Efficacy of marinades against listeria monocytogenes cells in suspension or associated ... 808048 - In vitro study of the influence of virginiamycin and spiramycin on the composition and ... 18164718 - Essential oil composition of valeriana officinalis l. roots cultivated in iran. compara... 3945968 - Hepatotoxic action of a poisonous mushroom, amanita abrupta in mice and its toxic compo... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-21 |
Journal Detail:
|
Title: Applied microbiology and biotechnology Volume: 89 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-01-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
|
Languages: eng Pagination: 73-8 Citation Subset: IM |
Affiliation:
|
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, #1800 Lihu Road, Wuxi 214122, JiangSu Province, People's Republic of China. xyge168@163.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nitroreductase activity of ferredoxin reductase BphA4 from Dyella ginsengisoli LA-4 by catalytic and...
Next Document: Recombinant antimicrobial peptide hPAB-? expressed in Pichia pastoris, a potential agent active agai...