| Improved vectors for expression library immunization--application to Mycoplasma hyopneumoniae infection in pigs. | |
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MedLine Citation:
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PMID: 11567754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Expression library immunization (ELI) has previously been used in a number of disease models in mice. Here, we describe the first example of the application of ELI to a large animal model with the immunization of pigs against enzootic pneumonia, a disease caused by Mycoplasma hyopneumoniae. The development of new plasmid vectors and library screening methods facilitated the application of ELI to this disease by allowing random libraries to be screened for clones expressing recombinant proteins. In this way the vast majority of clones in random libraries that are unproductive can be eliminated, meaning that libraries are more likely to give protection and are subsequently easier to further screen and analyze. By using this approach we have used one library screen and two animal trials to progress from an original library of 20,000 clones to a group of just 96 clones. |
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Authors:
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R J Moore; C Lenghaus; S A Sheedy; T J Doran |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Vaccine Volume: 20 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-09-24 Completed Date: 2002-01-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: England |
Other Details:
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Languages: eng Pagination: 115-20 Citation Subset: IM |
Affiliation:
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Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Vic. 3220, Geelong, Australia. robert.moore@li.csiro.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antigens, Bacterial / genetics*, immunology Base Sequence Escherichia coli Gene Library* Genetic Vectors / genetics*, immunology Molecular Sequence Data Mycoplasma / genetics, immunology* Pneumonia of Swine, Mycoplasmal / immunology, microbiology, prevention & control, veterinary* Swine Swine Diseases / immunology, microbiology, prevention & control* Vaccination / methods, veterinary* Vaccines, DNA / genetics, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Vaccines, DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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