| Improved techniques for examining rapid dopamine signaling with iontophoresis . | |
| | |
MedLine Citation:
|
PMID: 23276986 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Dopamine is a neurotransmitter that is utilized in brain circuits associated with reward processing and motor activity. Advances in microelectrode techniques and cyclic voltammetry have enabled its extracellular concentration fluctuations to be examined on a subsecond time scale in the brain of anesthetized and freely moving animals. The microelectrodes can be attached to micropipettes that allow local drug delivery at the site of measurement. Drugs that inhibit dopamine uptake or its autoreceptors can be evaluated while only affecting the brain region directly adjacent to the electrode. The drugs are ejected by iontophoresis in which an electrical current forces the movement of molecules by a combination of electrical migration and electroosmosis. Using electroactive tracer molecules, the amount ejected can be measured with cyclic voltammetry. In this review we will give an introduction to the basic principles of iontophoresis, including a historical account on the development of iontophoresis. It will also include an overview of the use of iontophoresis to study neurotransmission of dopamine in the rat brain. It will close by summarizing the advantages of iontophoresis and how the development of quantitative iontophoresis will facilitate future studies. |
| | |
Authors:
|
Natalie Rios Herr; Robert Mark Wightman |
Related Documents
:
|
16372476 - Oral administration of cyclosporin a in patients with severe alopecia areata. 15879096 - Cyclophilin a-deficient mice are resistant to immunosuppression by cyclosporine. 8836696 - Which drug for which orthostatic hypotension? 15560036 - Management of primary biliary cirrhosis and autoimmune cholangitis. 528856 - Toxicity of chelated mercury. 17027426 - Empirical-bayes adjustment improved conventional estimates in postmarketing drug-safety... |
Publication Detail:
|
Type: Journal Article Date: 2013-01-01 |
Journal Detail:
|
Title: Frontiers in bioscience (Elite edition) Volume: 5 ISSN: 1945-0508 ISO Abbreviation: Front Biosci (Elite Ed) Publication Date: 2013 |
Date Detail:
|
Created Date: 2013-01-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101485240 Medline TA: Front Biosci (Elite Ed) Country: United States |
Other Details:
|
Languages: eng Pagination: 249-57 Citation Subset: IM |
Affiliation:
|
The University of North Carolina at Chapel Hill, Department of Chemistry, C.B. 3290, Chapel Hill, N.C. 27599-3290. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regenerative strategies for preserving and restoring cardiac function .
Next Document: Cognitive impairment and dementia in bipolar disorder .