Document Detail


Improved techniques for examining rapid dopamine signaling with iontophoresis.
MedLine Citation:
PMID:  23276986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dopamine is a neurotransmitter that is utilized in brain circuits associated with reward processing and motor activity. Advances in microelectrode techniques and cyclic voltammetry have enabled its extracellular concentration fluctuations to be examined on a subsecond time scale in the brain of anesthetized and freely moving animals. The microelectrodes can be attached to micropipettes that allow local drug delivery at the site of measurement. Drugs that inhibit dopamine uptake or its autoreceptors can be evaluated while only affecting the brain region directly adjacent to the electrode. The drugs are ejected by iontophoresis in which an electrical current forces the movement of molecules by a combination of electrical migration and electroosmosis. Using electroactive tracer molecules, the amount ejected can be measured with cyclic voltammetry. In this review we will give an introduction to the basic principles of iontophoresis, including a historical account on the development of iontophoresis. It will also include an overview of the use of iontophoresis to study neurotransmission of dopamine in the rat brain. It will close by summarizing the advantages of iontophoresis and how the development of quantitative iontophoresis will facilitate future studies.
Authors:
Natalie Rios Herr; Robert Mark Wightman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2013-01-01
Journal Detail:
Title:  Frontiers in bioscience (Elite edition)     Volume:  5     ISSN:  1945-0508     ISO Abbreviation:  Front Biosci (Elite Ed)     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-06-05     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  101485240     Medline TA:  Front Biosci (Elite Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-57     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Corpus Striatum / physiology*
Dopamine / analysis*,  metabolism
Drug Delivery Systems / methods*
Iontophoresis / methods*
Microelectrodes
Rats
Synaptic Transmission / physiology*
Grant Support
ID/Acronym/Agency:
DA 10900/DA/NIDA NIH HHS; NS15841/NS/NINDS NIH HHS; R01 DA010900/DA/NIDA NIH HHS; R01 NS015841/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
VTD58H1Z2X/Dopamine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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