| Improved taxol yield in cell suspension culture of Taxus wallichiana (Himalayan yew) | |
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MedLine Citation:
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PMID: 9581525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell culture of Taxus wallichiana Zucc (= T. baccata ssp wallichiana Zucc. Pilg.) (Himalayan yew) has been established and taxol-producing cell lines selected by cell line cloning. Cell line NC110 derived from needle leaf of a 40-year-old tree growing in Darjeeling Himalayas produced 0.018% taxol in B5 basal medium supplemented with 2,4-D (2 mg/l), kinetin (0.5 mg/l), and casein hydrolysate (0.5%). This cell line has been maintained for over 2 years. Significant enhancement in the level of taxol (0.05%) was obtained in this cell line by supplementation of the basal medium with 5 mg/l of IAA-phenylalanine instead of 2,4-D without adversely affecting cell growth. IAA-glycine also enhanced taxol level (0.03%) while IAA alone (1-10 mg/l) was ineffective in inducing taxol accumulation. Using three different cell lines with different taxol-producing capacities, it has been demonstrated that 2,4-D and IAA-phenylalanine when present alone favour growth and taxol production but when combined enhance biomass to a maximum (six-fold in NC110) without enhancing taxol accumulation, suggesting that a two-stage culture may be beneficial for optimising taxol accumulation in cell culture of T. wallichiana. |
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Authors:
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S Jha; D Sanyal; B Ghosh; T B Jha |
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Publication Detail:
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Type: Letter; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Planta medica Volume: 64 ISSN: 0032-0943 ISO Abbreviation: Planta Med. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-06-18 Completed Date: 1998-06-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0066751 Medline TA: Planta Med Country: GERMANY |
Other Details:
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Languages: eng Pagination: 270-2 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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isolation & purification* Cells, Cultured Paclitaxel / isolation & purification* Trees |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 33069-62-4/Paclitaxel |
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