| Improved specificity of cardiolipin peroxidation by soybean lipoxygenase: a liquid chromatography - electrospray ionization mass spectrometry investigation. | |
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MedLine Citation:
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PMID: 22223416 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Peroxidation catalysed by Soybean Lypoxigenase was performed on tetralinoleyl-cardiolipin with the aim of generating selectively oxidized products, to be used subsequently as standards for studies on cardiolipin oxidation. The reaction products were characterized by LC-ESI-MS and MS/MS, and the process was found to link a hydroperoxylic group on one or more linoleic chains of cardiolipin, up to a total of four groups per molecule. Interestingly, the incidence of other oxidized products, like those arising from multiple hydroxylation or mixed hydroxylation-hydroperoxydation, previously observed after the chemical oxidation of the same cardiolipin, was found to be negligible. Moreover, evidences for the presence of the hydroperoxylic group(s) almost exclusively on carbon 13 of the linoleic chain(s) were obtained by MS/MS measurements. The enzymatic approach, integrated with a preparative separation step, which could be developed by adapting the chromatographic conditions adopted in the present work for analytical purposes, represents a promising strategy for the synthesis of highly specific mono- or multi-peroxidated derivatives of cardiolipins. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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I Losito; E Conte; B Introna; F M Megli; F Palmisano |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of mass spectrometry : JMS Volume: 46 ISSN: 1096-9888 ISO Abbreviation: J Mass Spectrom Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-01-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9504818 Medline TA: J Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 1255-62 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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Dipartimento di Chimica, Università degli Studi di Bari, Via E. Orabona 4, 70126, Bari, Italy; Centro Interdipartimentale SMART, Università degli Studi di Bari, 70126, Bari, Italy. losito@chimica.uniba.it. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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