Document Detail

Improved spatial resolution in the imaging of biological tissue using desorption electrospray ionization.
MedLine Citation:
PMID:  22706326     Owner:  NLM     Status:  Publisher    
Desorption electrospray ionization imaging allows biomarker discovery and disease diagnosis through chemical characterization of biological samples in their native environment. Optimization of experimental parameters including emitter capillary size, solvent composition, solvent flow rate, mass spectrometry scan-rate and step-size is shown here to improve the resolution available in the study of biological tissue from 180 μm to about 35 μm using an unmodified commercial mass spectrometer. Mouse brain tissue was used to optimize and measure resolution based on known morphological features and their known relationships to major phospholipid components. Features of approximately 35 μm were resolved and correlations drawn between features in grey matter (principally PS (18:0/22:6), m/z 834) and in white matter (principally ST (24:1), m/z 888). The improved spatial resolution allowed characterization of the temporal changes in lipid profiles occurring within mouse ovaries during the ovulatory cycle. An increase in the production of phosphatidylinositol (PI 38:4) m/z 885 and associated fatty acids such as arachidonic acid (FA 20:4) m/z 303 and adrenic acid (FA 22:4) m/z 331was seen with the postovulatory formation of the corpus luteum.
Dahlia I Campbell; Christina R Ferreira; Livia S Eberlin; R Graham Cooks
Related Documents :
20692666 - Determination of polyphenolic compounds by liquid chromatography-mass spectrometry in t...
21064146 - Colonic availability of polyphenols and d-(-)-quinic acid after apple smoothie consumpt...
23870056 - Two new ent-kaurane diterpenoids from pteris semipinnata.
22195836 - Synthesis of bioreducible and acid labile poly(amido amine)s via michael-addition react...
3163736 - Synthesis and hypolipidemic activity of 2-substituted isobutyric acid derivatives.
10224026 - Detection of salmonella spp. and listeria monocytogenes in suspended organic waste by n...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-16
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Chemistry Department, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  NMR metabolomics for assessment of exercise effects with mouse biofluids.
Next Document:  A double-blind randomized controlled trial of N-acetylcysteine in cannabis-dependent adolescents.