Document Detail


Improved spatial resolution in the imaging of biological tissue using desorption electrospray ionization.
MedLine Citation:
PMID:  22706326     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Desorption electrospray ionization imaging allows biomarker discovery and disease diagnosis through chemical characterization of biological samples in their native environment. Optimization of experimental parameters including emitter capillary size, solvent composition, solvent flow rate, mass spectrometry scan-rate and step-size is shown here to improve the resolution available in the study of biological tissue from 180 μm to about 35 μm using an unmodified commercial mass spectrometer. Mouse brain tissue was used to optimize and measure resolution based on known morphological features and their known relationships to major phospholipid components. Features of approximately 35 μm were resolved and correlations drawn between features in grey matter (principally PS (18:0/22:6), m/z 834) and in white matter (principally ST (24:1), m/z 888). The improved spatial resolution allowed characterization of the temporal changes in lipid profiles occurring within mouse ovaries during the ovulatory cycle. An increase in the production of phosphatidylinositol (PI 38:4) m/z 885 and associated fatty acids such as arachidonic acid (FA 20:4) m/z 303 and adrenic acid (FA 22:4) m/z 331was seen with the postovulatory formation of the corpus luteum.
Authors:
Dahlia I Campbell; Christina R Ferreira; Livia S Eberlin; R Graham Cooks
Related Documents :
14759156 - Ferulic acid release and 4-vinylguaiacol formation during brewing and fermentation: ind...
19071776 - Optimization of phenolic compounds analysis by capillary electrophoresis.
11958626 - Influence of cultivar on quality parameters and chemical composition of strawberry frui...
25408906 - Elevated oleic acid serum concentrations in patients suffering from alcohol dependence.
16463826 - Extracellular amino acid changes in patients during reversible cerebral ischaemia.
8934636 - Isolation from bovine liver mitochondria and characterization of three distinct carboxy...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-16
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Chemistry Department, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  NMR metabolomics for assessment of exercise effects with mouse biofluids.
Next Document:  A double-blind randomized controlled trial of N-acetylcysteine in cannabis-dependent adolescents.