Document Detail


Improved radioimmunoassay for creatine kinase isoenzymes in plasma.
MedLine Citation:
PMID:  7296839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe convenient and relatively rapid procedures for purifying creatine kinase isoenzymes MM, BB, and MB, and their use in an improved radioimmunoassay for creatine kinase isoenzymes in plasma. The modifications include use of: (a) BB with a specific activity of 400 kU/g, which can be labeled with a specific radioactivity of 20 Ci/g; (b) albumin-free purified MB as inhibitor; (c) antiserum to MB creatine kinase; and (d) a second-antibody technique that necessitates only a 15-min incubation. The radioimmunoassay for MB has a sensitivity of 0.2 microgram/L (80 mU/L) and a CV of less than 5%. Plasma MB average 22 (SD 12) microgram/L in 200 normal subjects; 24 (SD 12) microgram/L in 200 patients with chest pain without infarction; and 23 (SD 7) microgram/L in 43 patients with renal disease, whether measured before or after dialysis. Peak values for plasma MB averaged 191 (SD 86) microgram/L in 325 patients with documented myocardial infarction; BB was negligible. Extensive clinical experience indicates the radioimmunoassay to be suitably rapid, highly sensitive, and reliable as a diagnostic assay for MB in plasma.
Authors:
C S Ritter; S R Mumm; R Roberts
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  27     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-01-20     Completed Date:  1982-01-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1878-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Brain / enzymology
Clinical Enzyme Tests
Creatine Kinase / blood*,  isolation & purification
Humans
Isoenzymes
Kidney Diseases / enzymology
Kinetics
Myocardial Infarction / diagnosis,  enzymology
Myocardium / enzymology
Radioimmunoassay / methods
Grant Support
ID/Acronym/Agency:
HL 17646/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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