| Improved protocol for high-quality co-extraction of DNA and RNA from rumen digesta. | |
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MedLine Citation:
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PMID: 20680573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report an improved method for total nucleic acids extraction from rumen content samples. The method employs bead beating, and phenol-chloroform extraction followed by saline-alcohol precipitation. Total nucleic acids and RNA yield and purity were assessed by spectrophotometric measurements; RNA integrity was estimated using Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer. The method provided total nucleic acids and RNA extracts of good quantity and quality. The extraction is not time consuming and it is valuable for ecological studies of rumen microbial community structure and gene expression. |
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Authors:
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M Popova; C Martin; D P Morgavi |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-03 |
Journal Detail:
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Title: Folia microbiologica Volume: 55 ISSN: 1874-9356 ISO Abbreviation: Folia Microbiol. (Praha) Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376757 Medline TA: Folia Microbiol (Praha) Country: United States |
Other Details:
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Languages: eng Pagination: 368-72 Citation Subset: IM |
Affiliation:
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INRA Clermont-Ferrand-Theix, 63122, Saint Gènes Champanelle, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA / isolation & purification* Genomics / methods* Microbiological Techniques / methods* Molecular Biology / methods* RNA / isolation & purification* Rumen / microbiology* |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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