Document Detail

Improved postischemic ventricular functional recovery by amphetamine is linked with its ability to induce heat shock.
MedLine Citation:
PMID:  7845374     Owner:  NLM     Status:  MEDLINE    
Heat shock has been shown to increase the cellular tolerances to ischemic injury. In this study, we examined the effects of heat shock induced by amphetamine on postischemic myocardial functional recovery in a setting of coronary revascularization for acute myocardial infarction. Intramuscular injection of amphetamine (3 mg/kg, i.m.) to pigs increased the body temperature to 42.5 degrees C within 1 h, and maintained this temperature for an additional 2 h. Fourty h after the amphetamine injection, the pigs were placed on by cardiopulmonary bypass and then isolated, in situ heart preparations were subjected to 1 h of global hypothermic cardioplegic arrest and 1 h of normothermic reperfusion. Postischemic myocardial performance was monitored by measuring left ventricular (LV) pressure, its dp/dt, myocardial segmental shortening (%SS), and coronary blood flow. Cellular injury was examined by measuring creatine kinase (CK) release. Biochemical measurements included quantification of plasma catecholamines and study of the induction of heat shock gene expression and antioxidative enzymes in the heart tissue. The results of this study indicated significantly greater recovery of LV contractile functions by amphetamine as demonstrated by improved recovery of LVDP (61% vs 52%), dp/dtmax (52% vs 44%), and segmental shortening (46.2% vs 10%). Myocardial CK release was significantly reduced in the amphetamine group. Furthermore, amphetamine pretreatment was associated with the induction of heat shock protein (HSP) 27 mRNA and stimulated Cu/Zn-superoxide dismutase and catalase levels, suggesting that amphetamine mediated improved postischemic ventricular recovery might be linked with its ability to induce heat shock and stimulate antioxidant enzymes.
N Maulik; Z Wei; X Liu; R M Engelman; J A Rousou; D K Das
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  137     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1995-03-06     Completed Date:  1995-03-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  17-24     Citation Subset:  IM    
Department of Surgery, University of Connecticut School of Medicine, Farmington 06030-1110.
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MeSH Terms
Amphetamine / pharmacology,  therapeutic use*
Body Temperature / drug effects*
Cardiopulmonary Bypass
Catalase / biosynthesis,  genetics
Enzyme Induction / drug effects
Gene Expression Regulation / drug effects*
Heat-Shock Proteins / biosynthesis*,  genetics
Hot Temperature*
Hypothermia, Induced
Myocardial Infarction / physiopathology*
Myocardial Reperfusion Injury / pathology,  prevention & control*
RNA, Messenger / biosynthesis
Superoxide Dismutase / biosynthesis,  genetics
Ventricular Function, Left / drug effects*
Grant Support
Reg. No./Substance:
0/Heat-Shock Proteins; 0/RNA, Messenger; 300-62-9/Amphetamine; EC; EC Dismutase

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