| Improved postischemic ventricular functional recovery by amphetamine is linked with its ability to induce heat shock. | |
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MedLine Citation:
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PMID: 7845374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heat shock has been shown to increase the cellular tolerances to ischemic injury. In this study, we examined the effects of heat shock induced by amphetamine on postischemic myocardial functional recovery in a setting of coronary revascularization for acute myocardial infarction. Intramuscular injection of amphetamine (3 mg/kg, i.m.) to pigs increased the body temperature to 42.5 degrees C within 1 h, and maintained this temperature for an additional 2 h. Fourty h after the amphetamine injection, the pigs were placed on by cardiopulmonary bypass and then isolated, in situ heart preparations were subjected to 1 h of global hypothermic cardioplegic arrest and 1 h of normothermic reperfusion. Postischemic myocardial performance was monitored by measuring left ventricular (LV) pressure, its dp/dt, myocardial segmental shortening (%SS), and coronary blood flow. Cellular injury was examined by measuring creatine kinase (CK) release. Biochemical measurements included quantification of plasma catecholamines and study of the induction of heat shock gene expression and antioxidative enzymes in the heart tissue. The results of this study indicated significantly greater recovery of LV contractile functions by amphetamine as demonstrated by improved recovery of LVDP (61% vs 52%), dp/dtmax (52% vs 44%), and segmental shortening (46.2% vs 10%). Myocardial CK release was significantly reduced in the amphetamine group. Furthermore, amphetamine pretreatment was associated with the induction of heat shock protein (HSP) 27 mRNA and stimulated Cu/Zn-superoxide dismutase and catalase levels, suggesting that amphetamine mediated improved postischemic ventricular recovery might be linked with its ability to induce heat shock and stimulate antioxidant enzymes. |
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Authors:
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N Maulik; Z Wei; X Liu; R M Engelman; J A Rousou; D K Das |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 137 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1995-03-06 Completed Date: 1995-03-06 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 17-24 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Connecticut School of Medicine, Farmington 06030-1110. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amphetamine
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pharmacology,
therapeutic use* Animals Body Temperature / drug effects* Cardiopulmonary Bypass Catalase / biosynthesis, genetics Enzyme Induction / drug effects Female Gene Expression Regulation / drug effects* Heat-Shock Proteins / biosynthesis*, genetics Hot Temperature* Hypothermia, Induced Male Myocardial Infarction / physiopathology* Myocardial Reperfusion Injury / pathology, prevention & control* RNA, Messenger / biosynthesis Superoxide Dismutase / biosynthesis, genetics Swine Ventricular Function, Left / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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HL 22559/HL/NHLBI NIH HHS; HL 33899/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins; 0/RNA, Messenger; 300-62-9/Amphetamine; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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