| Improved native UV laser induced fluorescence detection for single cell analysis in poly(dimethylsiloxane) microfluidic devices. | |
| | |
MedLine Citation:
|
PMID: 16814305 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Single cell analytics is a key method in the framework of proteom research allowing analyses, which are not subjected to ensemble-averaging, cell-cycle or heterogeneous cell-population effects. Our previous studies on single cell analysis in poly(dimethylsiloxane) microfluidic devices with native label-free laser induced fluorescence detection [W. Hellmich, C. Pelargus, K. Leffhalm, A. Ros, D. Anselmetti, Electrophoresis 26 (2005) 3689] were extended in order to improve separation efficiency and detection sensitivity. Here, we particularly focus on the influence of poly(oxyethylene) based coatings on the separation performance. In addition, the influence on background fluorescence is studied by the variation of the incident laser power as well as the adaptation of the confocal volume to the microfluidic channel dimensions. Last but not least, the use of carbon black particles further enhanced the detection limit to 25 nM, thereby reaching the relevant concentration ranges necessary for the label-free detection of low abundant proteins in single cells. On the basis of these results, we demonstrate the first electropherogram from an individual Spodoptera frugiperda (Sf9) cell with native label-free UV-LIF detection in a microfluidic chip. |
| | |
Authors:
|
Wibke Hellmich; Dominik Greif; Christoph Pelargus; Dario Anselmetti; Alexandra Ros |
Related Documents
:
|
16719355 - Laser scanning cytometry: principles and applications. 11641145 - Peanut lectin binds to a subpopulation of mitochondria-rich cells in the rainbow trout ... 11259455 - Identification and purification of functional human beta-cells by a new specific zinc-f... 9659235 - Imaging bioluminescent indicators shows ca2+ and atp permeability thresholds in live ce... 19898535 - Cells in shearable and nonshearable regions of salmonella enterica serovar enteritidis ... 1963075 - Tyrosine phosphorylation of a gap junction protein correlates with inhibition of cell-t... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-30 |
Journal Detail:
|
Title: Journal of chromatography. A Volume: 1130 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2006 Oct |
Date Detail:
|
Created Date: 2006-10-02 Completed Date: 2006-11-22 Revised Date: 2009-01-15 |
Medline Journal Info:
|
Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 195-200 Citation Subset: IM |
Affiliation:
|
Experimental Biophysics and Applied Nanoscience, Physics Department, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Dimethylpolysiloxanes / chemistry* Electrophoresis, Microchip / instrumentation, methods* Female Fluorescence* Lasers Reproducibility of Results Spodoptera Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
|
0/Dimethylpolysiloxanes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enantioselectivity of vesicle-forming chiral surfactants in capillary electrophoresis. Role of the s...
Next Document: A method for the low-level (ng g(-1)) determination of perfluorooctanoate in paper and textile by li...