Document Detail

Improved myocardial preservation during cold storage using substrate enhancement.
MedLine Citation:
PMID:  1973349     Owner:  NLM     Status:  MEDLINE    
This study was undertaken to determine whether substrate enhancement with L-glutamate during periods of cold storage would improve ventricular function in transplanted hearts. Thirty-one rabbit hearts were rapidly excised and perfused with Krebs-Henseleit buffer (37 degrees C) on a Langendorff apparatus. They were arrested with hypothermic (4 degrees C), crystalloid, potassium (25 mEq/L) cardioplegia and stored at 3 degrees C for three hours, followed by reperfusion with Krebs-Henseleit buffer for one hour. Hearts were treated in one of several ways: Group 1 (n = 8) did not receive any L-glutamate and serve as controls; group 2 (n = 8) had L-glutamate (4 mmol/L) added to both the cardioplegic and reperfusate solutions; group 3 (n = 5) received L-glutamate only before ischemia; group 4 (n = 5) received L-glutamate only in the cardioplegic solution; and group 5 (n = 5) received L-glutamate only in the reperfusate. Hearts receiving L-glutamate in the reperfusate with or without its addition to the cardioplegic solution (groups 2 and 5) had the best recovery of the first derivative of positive and negative change in left ventricular peak systolic pressure and no significant changes in left ventricular compliance. Pretreatment with L-glutamate alone (group 3) resulted in no better recovery than in group 1 hearts. We conclude that addition of L-glutamate to reperfusate solutions after periods of cold storage for transplantation enhances the recovery of ventricular function.
C K Haan; H L Lazar; S Rivers; C Coady; R J Shemin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  50     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-08-13     Completed Date:  1990-08-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  80-5     Citation Subset:  AIM; IM    
Department of Cardiothoracic Surgery, Boston University Medical Center, Massachusetts.
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MeSH Terms
Blood Pressure / physiology
Cardiac Output / physiology
Cardioplegic Solutions / administration & dosage
Coronary Circulation / physiology
Cryopreservation / methods*
Glutamates / administration & dosage,  therapeutic use*
Glutamic Acid
Heart / physiology*
Heart Transplantation / methods*,  physiology
Hypothermia, Induced
Myocardial Contraction / physiology
Myocardial Reperfusion / methods
Time Factors
Tissue Preservation / methods*
Grant Support
507-RR-05487/RR/NCRR NIH HHS
Reg. No./Substance:
0/Cardioplegic Solutions; 0/Glutamates; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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