Document Detail

Improved molecular detection of Angiostrongylus cantonensis in mollusks and other environmental samples with a species-specific internal transcribed spacer 1-based TaqMan assay.
MedLine Citation:
PMID:  20543049     Owner:  NLM     Status:  MEDLINE    
Angiostrongylus cantonensis is the most common cause of human eosinophilic meningitis. Humans become infected by ingesting food items contaminated with third-stage larvae that develop in mollusks. We report the development of a real-time PCR assay for the species-specific identification of A. cantonensis in mollusk tissue.
Yvonne Qvarnstrom; Ana Cristina Aramburu da Silva; John L Teem; Robert Hollingsworth; Henry Bishop; Carlos Graeff-Teixeira; Alexandre J da Silva
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-11
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-11-01     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5287-9     Citation Subset:  IM    
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30329-4018, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GU587745;  GU587746;  GU587747;  GU587748;  GU587749;  GU587750;  GU587751;  GU587752;  GU587753;  GU587754;  GU587755;  GU587756;  GU587757;  GU587758;  GU587759;  GU587760;  GU587761;  GU587762;  GU733321;  GU733322;  GU733323;  GU733324;  GU733325
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MeSH Terms
Angiostrongylus cantonensis / genetics,  isolation & purification*
DNA Primers / genetics
DNA, Helminth / chemistry,  genetics
DNA, Ribosomal Spacer / chemistry,  genetics
Environmental Microbiology*
Molecular Sequence Data
Mollusca / parasitology*
Parasitology / methods*
Polymerase Chain Reaction / methods*
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA Primers; 0/DNA, Helminth; 0/DNA, Ribosomal Spacer

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