| Improved methods for the determination of 5-bromo-2-deoxyuridine labelling index in clones and colonies growing in plasma clots. | |
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MedLine Citation:
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PMID: 2337406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bromodeoxyuridine (BrdUrd), an analogue of thymidine is one of the most employed marker to detect the S-phase of the cell cycle. Difficulties are described for the in situ detection of S-phase cells in normal and neoplastic growing clones. In this paper we propose new methods for the detection of BrdUrd in neoplastic clones, growing in plasma clots. In particular, these methods are based on the immunocytochemical staining with horseradish peroxidase and alkaline phosphatase, as well as on a new immunofluorescent streptavidin technique. They allow easy detection of S-phase cells with an inverted light microscope. |
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Authors:
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G D Di Nucci; P L Tazzari; A Bontadini; C Tassi; A Dinota; S Pileri; M Gobbi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Basic and applied histochemistry Volume: 34 ISSN: 0391-7258 ISO Abbreviation: Basic Appl Histochem Publication Date: 1990 |
Date Detail:
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Created Date: 1990-06-14 Completed Date: 1990-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7910664 Medline TA: Basic Appl Histochem Country: ITALY |
Other Details:
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Languages: eng Pagination: 71-7 Citation Subset: IM |
Affiliation:
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Dipartimento di Biopatologia Umana, Università degli Studi La Sapienza, Roma, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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diagnostic use* Bromodeoxyuridine / diagnostic use, pharmacokinetics* Cell Cycle Cell Line Culture Techniques / methods* Humans Immunohistochemistry Plasma* Tumor Cells, Cultured / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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