Document Detail


Improved method of superovulation in monovulatory brushtail possums (Trichosurus vulpecula) using pregnant mares' serum gonadotrophin-luteinizing hormone.
MedLine Citation:
PMID:  9861159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new superovulation regimen for the monovulatory brushtail possum (Trichosurus vulpecula) has been devised. It reduces the number of hormone treatments required and elicits a better rate of ovulation than the established pregnant mares' serum gonadotrophin (PMSG)-GnRH method. Ovarian stimulation was achieved by a single intramuscular injection of 15 iu PMSG. This treatment resulted in the recruitment and development of a large number (9-12) of Graafian follicles on the ovaries. Induction of ovulation was achieved by a single intramuscular injection of 2-10 mg pig LH or multiple intramuscular injections of GnRH, (4 x 50 micrograms, 90 min apart) given 72 h after PMSG treatment. Superovulation occurred in all animals (n = 48) examined 48 h after treatment irrespective of dose of LH or type of ovulatory stimulus used. The highest ovulatory success (83%), the maximum number of ovulation sites (9.5 +/- 2.8) and the highest number of oocytes recovered (9.0 +/- 2.5) were achieved after treatment with PMSG and 4 mg LH. These results were significantly greater than the ovulatory success (43%), numbers of ovulation sites (3.9 +/- 1.1) and number of oocytes recovered (2.1 +/- 0.9) after PMSG-GnRH treatment (P < 0.05). This simpler and more effective superovulation protocol should assist with more effective manipulation of possum reproduction in captivity.
Authors:
A M Glazier; F C Molinia
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  113     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1999-01-12     Completed Date:  1999-01-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-5     Citation Subset:  IM    
Affiliation:
Cooperative Research Centre for Conservation and Management of Marsupials at Manaaki, Canterbury, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Evaluation Studies as Topic
Female
Gonadotropin-Releasing Hormone / administration & dosage
Gonadotropins, Equine / administration & dosage*
Injections, Intramuscular
Luteinizing Hormone / administration & dosage*
Oocytes / cytology,  drug effects
Opossums*
Ovary / anatomy & histology,  drug effects
Superovulation / drug effects*
Chemical
Reg. No./Substance:
0/Gonadotropins, Equine; 33515-09-2/Gonadotropin-Releasing Hormone; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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