Document Detail

Improved method for measurement of dietary fiber as non-starch polysaccharides in plant foods.
MedLine Citation:
PMID:  2458334     Owner:  NLM     Status:  MEDLINE    
A method is described that allows rapid estimation of total, soluble, and insoluble dietary fiber as the non-starch polysaccharides (NSP) in plant foods. It is a modification of an earlier, more complex procedure. Starch is completely removed enzymatically, and NSP is measured as the sum of its constituent sugars released by acid hydrolysis. The sugars may, in turn, be measured by gas chromatography (GC), giving values for individual monosaccharides, or more rapidly by colorimetry. Both GC and colorimetry are suitable for routine measurement of total, soluble, and insoluble dietary fiber in cereals, fruits, and vegetables. Values obtained are not affected by food processing so the dietary fiber content of various processed foods and mixed diets can be calculated simply from knowing the amount in the raw materials. The additional information obtained by GC analysis is valuable in the interpretation of physiological studies and in epidemiology where disease is related to type and amount of dietary fiber.
H N Englyst; J H Cummings
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  71     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1988 Jul-Aug
Date Detail:
Created Date:  1988-10-26     Completed Date:  1988-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  808-14     Citation Subset:  IM    
M.R.C. Dunn Clinical Nutrition Centre, Cambridge, U.K.
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MeSH Terms
Amylases / analysis
Carbohydrates / analysis
Dietary Fiber / analysis*
Food Analysis*
Fruit / analysis*
Indicators and Reagents
Polysaccharides / analysis*
Starch / analysis
Uronic Acids / analysis
Vegetables / analysis*
Reg. No./Substance:
0/Carbohydrates; 0/Indicators and Reagents; 0/Polysaccharides; 0/Uronic Acids; 9005-25-8/Starch; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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