| Improved method for measurement of dietary fiber as non-starch polysaccharides in plant foods. | |
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MedLine Citation:
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PMID: 2458334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method is described that allows rapid estimation of total, soluble, and insoluble dietary fiber as the non-starch polysaccharides (NSP) in plant foods. It is a modification of an earlier, more complex procedure. Starch is completely removed enzymatically, and NSP is measured as the sum of its constituent sugars released by acid hydrolysis. The sugars may, in turn, be measured by gas chromatography (GC), giving values for individual monosaccharides, or more rapidly by colorimetry. Both GC and colorimetry are suitable for routine measurement of total, soluble, and insoluble dietary fiber in cereals, fruits, and vegetables. Values obtained are not affected by food processing so the dietary fiber content of various processed foods and mixed diets can be calculated simply from knowing the amount in the raw materials. The additional information obtained by GC analysis is valuable in the interpretation of physiological studies and in epidemiology where disease is related to type and amount of dietary fiber. |
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Authors:
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H N Englyst; J H Cummings |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal - Association of Official Analytical Chemists Volume: 71 ISSN: 0004-5756 ISO Abbreviation: J Assoc Off Anal Chem Publication Date: 1988 Jul-Aug |
Date Detail:
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Created Date: 1988-10-26 Completed Date: 1988-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7505559 Medline TA: J Assoc Off Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 808-14 Citation Subset: IM |
Affiliation:
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M.R.C. Dunn Clinical Nutrition Centre, Cambridge, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Amylases / analysis Carbohydrates / analysis Colorimetry Dietary Fiber / analysis* Food Analysis* Fruit / analysis* Hydrolysis Indicators and Reagents Oxidation-Reduction Polysaccharides / analysis* Starch / analysis Uronic Acids / analysis Vegetables / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Indicators and Reagents; 0/Polysaccharides; 0/Uronic Acids; 9005-25-8/Starch; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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