Document Detail


Improved method for the determination of phospholipase A2 catalytic activity concentration in human serum and ascites.
MedLine Citation:
PMID:  3694138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved radiochemical method is presented for the selective determination of phospholipase A2 activity in human serum and ascites, using only commercially available reagents. The method can be applied to large quantities of samples. As substrate we used 1,2-dipalmitoyl-sn-glycero(3)phosphorylcholine and phosphatidylcholine containing tritiated palmitic acid in position 2 (1-palmitoyl,2-[9,10-3H]palmitoyl-sn-glycero(3)phosphorylcholine). The liberated fatty acids are extracted and radioactivity is detected in a liquid scintillation spectrometer. A preliminary reference range of human serum samples was established ranging up to 1.0 U/l. In sera of patients with acute pancreatitis we found activities up to 20 U/l. The correlation of phospholipase A2 activity with that of other enzymes and with the severity and complications of acute pancreatitis was investigated. A possible relationship between phospholipase A2 activities and pulmonary complications is discussed.
Authors:
H R Schädlich; M Büchler; H G Beger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie     Volume:  25     ISSN:  0340-076X     ISO Abbreviation:  J. Clin. Chem. Clin. Biochem.     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1988-02-12     Completed Date:  1988-02-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7701860     Medline TA:  J Clin Chem Clin Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  505-9     Citation Subset:  IM    
Affiliation:
Abteilung für Allgemeine Chirurgie, Universität Ulm.
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MeSH Terms
Descriptor/Qualifier:
Ascites / enzymology*
Catalysis
Humans
Indicators and Reagents
Kinetics
Palmitic Acids / metabolism
Pancreas / enzymology
Pancreatitis / enzymology
Phospholipases / blood*
Phospholipases A / blood*
Phospholipases A2
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Palmitic Acids; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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