Document Detail


Improved method for the determination of furosine in food by capillary electrophoresis.
MedLine Citation:
PMID:  9798165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previously described method for determination of furosine by capillary zone electrophoresis in food has been improved for greater accuracy and sensitivity. Acid-hydrolyzed samples are subjected to solid phase extraction; then they are dried and redissolved before injection. The electrophoresis separation is performed in a fused silica capillary tube (50 microns inside diameter) with an extended-path-length detection cell using a 3-(N-morpholino)-2-hydroxypropanesulfonic acid solution at pH 7.0 as run buffer. The in-laboratory repeatability was within +/- 7.1% at the 95% confidence level when the amount of furosine ranged between 8 and 250 mg per 100 g protein. The values of both repeatability and sensitivity fulfill the requirements stated in the EU and Italian regulations for furosine determination in pasteurized milk and Mozzarella cheese. The data obtained on 48 different food samples including heat-treated milk, cheeses, and durum wheat products were comparable with those obtained by an HPLC method and proved to be accurate for furosine values up to at least 400 mg per 100 g protein. The furosine values found in cultured fresh cheese samples suggest that the use of this furosine index holds promise for the characterization of this type of cheese.
Authors:
A Tirelli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  61     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-01-08     Completed Date:  1999-01-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1400-4     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche (DI.S.T.A.M.), Università degli Studi di Milano, Italy. antonio.tirelli@unimi.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Cheese / analysis
Chromatography, High Pressure Liquid
Electrophoresis, Capillary / methods*
Food Analysis / methods*
Hydrochloric Acid
Hydrolysis
Lysine / analogs & derivatives*,  analysis
Milk / chemistry
Triticum / chemistry
Chemical
Reg. No./Substance:
19746-33-9/furosine; 56-87-1/Lysine; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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