Document Detail

Improved method for the determination of chlorhexidine in urine.
MedLine Citation:
PMID:  6715468     Owner:  NLM     Status:  MEDLINE    
A high-performance liquid chromatographic method is described which is suitable for the determination of the common antiseptic chlorhexidine in urine. The method employs Sep-Pak cartridges to remove chlorhexidine from the urine matrix. Chromatographic separation was achieved on a C18 reversed-phase column using a mobile phase of methanol-20 mM sodium acetate solution (60:40) adjusted to pH 5 with glacial acetic acid. An ion-pair agent (pentadecafluorooctanoic acid) was used at a concentration of 100 micrograms ml-1 X 3-Bromobenzophenone was used as chromatographic standard (kappa' = 4.0). 4-Bromobenzophenone (kappa' = 3.9) or dibenzal hydrazine (kappa' = 4.4) may also be used. A series of urine samples was analysed and no interferences were observed. The method is simple and rapid with a total analysis time of ca. 30 min.
M H Gaffney; M Cooke; R Simpson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  306     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-06-14     Completed Date:  1984-06-14     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  303-13     Citation Subset:  IM    
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MeSH Terms
Chemistry, Physical
Chlorhexidine / urine*
Chromatography, High Pressure Liquid / methods
Hydrogen-Ion Concentration
Physicochemical Phenomena
Spectrophotometry, Ultraviolet / methods
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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