| Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture. | |
| | |
MedLine Citation:
|
PMID: 22903442 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVE: To investigate whether electroacupuncture (EA) can promote cell survival and enhance heart function of mesenchymal stem cells (MSCs) therapy. METHODS: MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha (α-MEM). MI was induced in 72 Sprague-Dawley (S-D) rats by ligation of the left anterior descending coronary artery (LAD) for 30 min and reperfusion. MI rats randomly received injection of 1×10(6) DiI-labeled MSCs alone (n=24, MSC group), or plus electroacupuncture (EA) at Neiguan (PC6, n=24, EA+MSC group), or saline (n=24, saline group). EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment (sham group). Three time points, 4, 14 and 28 days (n=8 for each group) were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot at day 4 and 14. Left ventricular remodeling, cardiac function, infarction area, fibrosis and capillary density were analyzed at day 28. RESULTS: EA can enhance MSC survival (2.6-fold up) at day 4. Big capillary density was 53% higher in EA+MSC treated group than MSC alone group. Furthermore, the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4. CONCLUSION: EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases. |
| | |
Authors:
|
Jin Zhang; Xiao-Hua Jia; Zhi-Wei Xu; Fu-Ping Ding; Xin Zhou; Hao Fu; Yi Liu; Lai-Liang Ou; Zong-Jin Li; De-Ling Kong |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-8-18 |
Journal Detail:
|
Title: Chinese journal of integrative medicine Volume: - ISSN: 1672-0415 ISO Abbreviation: Chin J Integr Med Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-8-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101181180 Medline TA: Chin J Integr Med Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin, 300071, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The absorption and transport of magnolol in Caco-2 cell model.
Next Document: Advising on preferred reporting items for patient-reported outcome instrument development: the PRIPR...