Document Detail


Improved media for growth and aerotolerance of Campylobacter fetus.
MedLine Citation:
PMID:  670386     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4 7H2O, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% O2, 17% O2, and 21% O2, respectively. Unsupplemented brucella agar supported growth of 94, 48, and 20% of the strains. Brucella broth supplemented with 0.2% FeSO4.7H2O, 0.025% sodium metabisulfite, and 0.05% sodium pyruvate supported growth of 98% of the strains at 21% O2, compared to 75% with unsupplemented brucella broth. With both the supplemented agar and broth, growth responses occurred 1 to 2 days earlier than usual. Growth and aerotolerance of three strains of Campylobacter sputorum subsp. bubulus were not enhanced by the supplements.
Authors:
H A George; P S Hoffman; R M Smibert; N R Krieg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  8     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1978 Jul 
Date Detail:
Created Date:  1978-09-29     Completed Date:  1978-09-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  36-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Campylobacter / growth & development*
Campylobacter fetus / growth & development*
Culture Media*
Ferrous Compounds
Oxygen*
Partial Pressure
Pyruvates
Sulfates
Sulfites
Chemical
Reg. No./Substance:
0/Culture Media; 0/Ferrous Compounds; 0/Pyruvates; 0/Sulfates; 0/Sulfites; 7782-44-7/Oxygen
Comments/Corrections

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