Document Detail


Improved liquid chromatography-tandem mass spectrometry method for the analysis of eptifibatide in human plasma.
MedLine Citation:
PMID:  20599442     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid, selective and highly sensitive high performance liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of eptifibatide in human plasma. Eptifibatide and the internal standard (IS), EPM-05, were extracted from plasma samples using solid phase extraction. Chromatographic separation was performed on a C(18) column at a flow rate of 0.5 mL/min. Detection of eptifibatide and the IS was achieved by tandem mass spectrometry with an electrospray ionization (ESI) interface in positive ion mode. Traditional multiple reaction monitoring (MRM) using the transition of m/z 832.6-->m/z 646.4 and m/z 931.6-->m/z 159.4 was performed to quantify eptifibatide and the IS, respectively. The calibration curves were linear over the range of 1-1000 ng/mL with the lower limit of quantitation validated at 1 ng/mL. The intra- and inter-day precisions were within 13.3%, while the accuracy was within +/-7.6% of nominal values. The validated LC-MS/MS method was successfully applied for the evaluation of pharmacokinetic parameters of eptifibatide after intravenous (i.v.) administration of a 45 microg/kg bolus of eptifibatide to 8 healthy volunteers.
Authors:
Zhi-Ling Zhou; Xi-Yong Yu; Min Yang; Li-Ping Mai; Qiu-Xiong Lin; Chun-Yu Deng; Zhi-Xin Shan; Su-Juan Kuang; Ping Zhu; Xiao-Zhong Huang; Xiao-Hong Li; Tie-Feng Chen; Shu-Guang Lin
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-18
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  878     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-21     Completed Date:  2010-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2094-100     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Medical Research Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
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MeSH Terms
Descriptor/Qualifier:
Asian Continental Ancestry Group
Chromatography, Liquid
Drug Stability
Female
Health
Humans
Male
Peptides / blood*,  chemistry,  pharmacokinetics
Platelet Aggregation Inhibitors / blood*,  chemistry,  pharmacokinetics
Reference Standards
Reproducibility of Results
Tandem Mass Spectrometry / methods*
Time Factors
Chemical
Reg. No./Substance:
0/Peptides; 0/Platelet Aggregation Inhibitors; 0/eptifibatide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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