| Improved liquid-chromatographic determination of cyclosporine, with concomitant detection of a cell-bound metabolite. | |
| | |
MedLine Citation:
|
PMID: 3967349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This unique extraction and isocratic "high-performance" liquid chromatographic method for measuring cyclosporine (CsA) in blood involves a Zorbax cyanopropyl analytical column maintained at 58 degrees C, with detection at 214 nm, and recycling of the water:acetonitrile mobile phase for improved long-term column stability and efficiency. Routinely, 1.0 mL of serum, plasma, or whole blood is diluted with water:acetonitrile (70:30) and applied to a disposable solid-phase cyanopropyl column to rapidly extract the drug and the internal standard cyclosporin D (CsD). Analytical recovery for this step averages 90% with whole blood and 98% with serum and plasma. Between-run CVs were 6.5 and 2.6% for means of 104 and 1128 micrograms/L, respectively. The standard curve is linear up to 1600 micrograms/L. The minimum detection limit is 10 to 15 micrograms/L. No interferences from endogenous substances or other drugs were found. In addition, a compound cross reacting with the Sandoz radioimmunoassay antibody was isolated from patients' samples with the present procedure and was tentatively identified as a CsA metabolite(s). It appears to be highly partitioned on blood cells, very little being detected in the serum or plasma. In a comparison with RIA, correlation coefficients were 0.828 and 0.652 for serum and whole blood, respectively. Results from a 12-h pharmacokinetic study in which different sample types were analyzed by RIA and liquid chromatography further exemplified major discrepancies between types of CsA determinations. |
| | |
Authors:
|
G L Lensmeyer; B L Fields |
Related Documents
:
|
12243909 - Detection of hantavirus infection in hemolyzed mouse blood using alkaline phosphatase c... 7663709 - High-performance liquid chromatographic determination of diadenosine 5',5'"-p1,p4-tetra... 12296459 - On-line monitoring of solutes in dialysate using absorption of ultraviolet radiation: t... 11234809 - Sensitive determination of n-hexane and cyclohexane in human body fluids by capillary g... 3054319 - Direct detection of drugs of abuse in whole hemolyzed blood using the emit d.a.u. urine... 23696609 - Metabolomic changes in the rat retina after optic nerve crush. 15762749 - Evolutionary chemistry approach toward finding novel inhibitors of the type 2 diabetes ... 11317709 - Use of solvents in industries in korea: experience in sinpyeong-jangrim industrial comp... 18961309 - Trace analysis by microwave excitation of sealed samples-i: preliminary investigations. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Clinical chemistry Volume: 31 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1985 Feb |
Date Detail:
|
Created Date: 1985-03-07 Completed Date: 1985-03-07 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 196-201 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blood Cells
/
metabolism Chromatography, High Pressure Liquid / methods Cyclosporins / blood* Humans Indicators and Reagents Plasma / metabolism Radioimmunoassay Solvents |
| Chemical | |
Reg. No./Substance:
|
0/Cyclosporins; 0/Indicators and Reagents; 0/Solvents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A more specific, simpler radioimmunoassay for carcinoembryonic antigen, with use of monoclonal antib...
Next Document: Computer evaluation of statistical procedures, and a new quality-control statistical procedure.