Document Detail

Improved light sources for induction of sister chromatid differentiation.
MedLine Citation:
PMID:  2419042     Owner:  NLM     Status:  MEDLINE    
Various light sources, including ultraviolet light, mercury, germicidal, fluorescent, and incandescent lamps, were studied for their ability to induce sister chromatid differentiation (SCD) in rat bone marrow cells. The light sources were used along with Hoechst 33258 and Giemsa stains for SCD induction. When those lamps which emit significant amounts of heat were used, 60 degrees C incubation in 2X SSC was found to be unnecessary for SCD induction. A high wattage lamp, a high ambient temperature, a short distance between the lamp and the slides, or a light with 360 nm wavelength, minimized the required exposure time to the light. The pH value of the mounting buffer was also a significant factor. Fluorescent black light and incandescent lamps were found to be ideal light sources for SCD induction.
J F Chen; Y J Lin
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  44     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1985  
Date Detail:
Created Date:  1986-04-02     Completed Date:  1986-04-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  73-87     Citation Subset:  IM    
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MeSH Terms
Bone Marrow Cells
Disinfection / instrumentation
Hydrogen-Ion Concentration
Light / diagnostic use*
Luminescent Measurements
Rats, Inbred Strains
Sister Chromatid Exchange / radiation effects*
Staining and Labeling
Time Factors
Ultraviolet Rays
Reg. No./Substance:
0/Buffers; 23491-44-3/Bisbenzimidazole; 7439-97-6/Mercury

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