| Improved light microscopic demonstration of D-amino acid oxidase activity in cryotome sections using cerium ions as capturing and amplifying agent--the Ce/Ce-H2O2-DAB procedure. | |
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MedLine Citation:
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PMID: 1682047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The light microscopical demonstration of D-amino acid oxidase (AAOX) activity with cerium (Ce III) as the capturing agent was improved. The incubation medium was stabilized by the employment of triethanolamine and detrane complexed cerium. A considerable increase in intensity of the reaction was accomplished by treatment of the AAOX-incubated sections with Ce III which reacted with the primary reaction product Ce IV-perhydroxide to form Ce IV-hydroxide. In this way the primary reaction product was reduced and enlarged concomitantly. The Ce IV-hydroxide was converted into Ce IV-perhydroxide by H2O2, which was visualized by blue-black stained Ni-DAB complexes. Thus, Ce III is used as capturing agent as well as amplifier (Ce/Ce-H2O2-DAB method). The primary reaction product Ce III-phosphate formed by coreacting phosphatases was selectively extracted by citrate containing glycine-NaOH buffer while Ce IV-perhydroxide remained in the sections. In model experiments it was proven that the perhydroxide groups in the Ce IV-perhydroxide compound initiate predominantly the DAB polymerization while the contribution of Ce III and Ce IV is small. |
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Authors:
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K J Halbhuber; H Feuerstein; N Zimmermann; M Klinger; D Kalicharan; U Hupfer |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cellular and molecular biology Volume: 37 ISSN: 0145-5680 ISO Abbreviation: Cell. Mol. Biol. Publication Date: 1991 |
Date Detail:
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Created Date: 1991-12-03 Completed Date: 1991-12-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7801029 Medline TA: Cell Mol Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 279-94 Citation Subset: IM |
Affiliation:
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Department of Histochemistry, Friedrich Schiller University Jena, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3,3'-Diaminobenzidine Animals Buffers Cerium* Citrates / pharmacology D-Amino-Acid Oxidase / analysis* Female Freezing Histological Techniques* Hydrogen Peroxide Male Microscopy / methods* Oxidation-Reduction Rats Rats, Inbred Strains Tissue Fixation |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Citrates; 7440-45-1/Cerium; 7722-84-1/Hydrogen Peroxide; 91-95-2/3,3'-Diaminobenzidine; EC 1.4.3.3/D-Amino-Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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