Document Detail


Improved insulin receptor assay: effects of an antidiabetic sulphonylurea on liver membrane insulin receptors from obese hyperglycaemic mice.
MedLine Citation:
PMID:  465886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1 A simple assay of liver membrane insulin receptors is described, under conditions which apparently stabilized the insulin-receptor complex. The receptor population was measured in the presence of physiological doses of radioactive hormone under conditions which minimized negative co-operativity effects. 2 Equilibrium between receptor and hormone was attained in vitro within 60 min of incubation time at 13 degrees C with no apparent loss of binding sites after 3 h of incubation. 3 Liver membranes from lean mice possessed 8.8 times the receptor number per mg membrane protein measured in membranes from obese hyperglycaemic (ob/ob) littermates. After treatment of ob/ob mice with ARDF-26 (Gliquidon; Boehringer Ingelheim Ltd) the concentration of liver membrane insulin receptors was increased 5 fold.
Authors:
B D Greenstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  66     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-10-26     Completed Date:  1979-10-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  317-22     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / drug effects,  metabolism
Hyperglycemia / metabolism*
Hypoglycemic Agents / pharmacology*
Insulin / metabolism
Kinetics
Liver / metabolism*
Male
Mice
Mice, Obese
Receptor, Insulin / analysis*,  drug effects,  metabolism
Sulfonylurea Compounds / pharmacology*
Chemical
Reg. No./Substance:
0/Hypoglycemic Agents; 0/Sulfonylurea Compounds; 11061-68-0/Insulin; EC 2.7.10.1/Receptor, Insulin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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