Document Detail

Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules.
MedLine Citation:
PMID:  17223139     Owner:  NLM     Status:  MEDLINE    
Using the chick chorioallantoic membrane assay (CAM) and a novel histological technique, we investigated the ability of blood vessels to directly invade fibrin-based scaffolds. In our initial experiments utilizing vascular endothelial growth factor (VEGF(165)), we found no direct invasion. Instead, the fibrin was completely degraded and replaced with highly vascularized new tissue. Addition of fibroblast growth factor-2 (FGF-2), bone morphogenic protein-2 (BMP-2), or platelet-derived growth factor-BB (PDGF-BB) to the fibrin construct also did not result in construct vascularization. Because natural and regenerating tissues exhibit complex extracellular matrices (ECMs), we hypothesized that a more complex scaffold may improve blood vessel invasion. Addition of fibronectin, hyaluronic acid, and collagen type I within 20 mg/mL fibrin constructs resulted in no significant improvement. However, the same additive concentrations within 10 mg/mL fibrin constructs resulted in dramatic improvements, specifically with hyaluronic acid. Overall, we believe that these results indicate the importance of structural and functional cues of not only in the initial scaffold but also as the construct is degraded and remodeled. Furthermore, the CAM assay may represent a useful model for understanding ECM interactions as well as for screening and designing tissue-engineered scaffolds.
J D Smith; M E Melhem; K T Magge; A S Waggoner; P G Campbell
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-01-16
Journal Detail:
Title:  Microvascular research     Volume:  73     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-19     Completed Date:  2007-04-24     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  84-94     Citation Subset:  IM    
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MeSH Terms
Allantois / blood supply
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / pharmacology
Chick Embryo
Chorion / blood supply
Collagen Type I / pharmacology
Extracellular Matrix Proteins / pharmacology*
Fibroblast Growth Factor 2 / pharmacology
Fibronectins / pharmacology
Growth Substances / pharmacology*
Hyaluronic Acid / pharmacology
Microscopy, Electron, Scanning
Neovascularization, Physiologic / drug effects*
Platelet-Derived Growth Factor / pharmacology
Proto-Oncogene Proteins c-sis
Recombinant Proteins / pharmacology
Transforming Growth Factor beta / pharmacology
Vascular Endothelial Growth Factor A / pharmacology
Grant Support
1R01 EB 00364-01/EB/NIBIB NIH HHS; 1T32 EB 00424-03/EB/NIBIB NIH HHS; R01 EB004343/EB/NIBIB NIH HHS; R01 EB004343-03/EB/NIBIB NIH HHS
Reg. No./Substance:
0/BMP2 protein, human; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Proteins; 0/Collagen Type I; 0/Extracellular Matrix Proteins; 0/Fibronectins; 0/Gels; 0/Growth Substances; 0/Platelet-Derived Growth Factor; 0/Proto-Oncogene Proteins c-sis; 0/Recombinant Proteins; 0/Transforming Growth Factor beta; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; 0/platelet-derived growth factor BB; 103107-01-3/Fibroblast Growth Factor 2; 9001-31-4/Fibrin; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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