Document Detail

Improved functional recovery to I/R injury in hearts from lipocalin-2 deficiency mice: restoration of mitochondrial function and phospholipids remodeling.
MedLine Citation:
PMID:  22355443     Owner:  NLM     Status:  PubMed-not-MEDLINE    
AIMS: Recent clinical and experimental evidences demonstrate an association between augmented circulating lipocalin-2 [a pro-inflammatory adipokine] and cardiac dysfunction. However, little is known about the pathophysi-ological role of lipocalin-2 in heart. The present study was designed to compare the heart functions of mice with normal (WT) or deficient lipocalin-2 (Lcn2-KO) expression.
METHODS AND RESULTS: Echocardiographic analysis revealed that the myocardial contractile function was significantly improved in hearts of Lcn2-KO mice, under both standard chow and high fat diet conditions. The heart function before and after I/R injury (20-min of global ischemia followed by 60-min of reperfusion) was assessed using the Langendorff perfusion system. Compared to WT littermates, hearts from Lcn2-KO mice showed improved functional recovery and reduced infarct size following I/R. Under baseline condition, the mitochondrial function of Lcn2-KO hearts was significantly enhanced, as demonstrated by biochemical analysis of respiratory chain activity and markers of biogenesis, as well as electron microscopic investigation of the mitochondrial ultrastructure. Acute or chronic administration of lipocalin-2 impaired cardiac functional recovery to I/R and dampened the mitochondrial function in hearts of Lcn2-KO mice. These effects were associated with an extensive modification of the fatty acyl chain compositions of intracellular phospholipids. For example, lipocalin-2 facilitated the redistribution of linoleic acid (C18:2) among different types of phospholipids, including cardiolipin, a structurally unique phospholipid located mainly on the inner membrane of mitochondria.
CONCLUSIONS: Lack of lipocalin-2 improved the functional recovery of isolated mice hearts subjected to I/R, which is associated with restoration of mitochondrial function and phospholipids remodeling.
Bo Yang; Pengcheng Fan; Aimin Xu; Karen Sl Lam; Thorsten Berger; Tak W Mak; Hung-Fat Tse; Jessie Ws Yue; Erfei Song; Paul M Vanhoutte; Gary Sweeney; Yu Wang
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Publication Detail:
Type:  Journal Article     Date:  2012-01-05
Journal Detail:
Title:  American journal of translational research     Volume:  4     ISSN:  1943-8141     ISO Abbreviation:  Am J Transl Res     Publication Date:  2012  
Date Detail:
Created Date:  2012-02-22     Completed Date:  2012-10-02     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101493030     Medline TA:  Am J Transl Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-71     Citation Subset:  -    
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