Document Detail

Improved fluorescence polarization assay for use in evaluating fetal lung maturity. III. Retrospective clinical evaluation and comparison with the lecithin/sphingomyelin ratio.
MedLine Citation:
PMID:  3943183     Owner:  NLM     Status:  MEDLINE    
We clinically evaluated, retrospectively, our improved fluorescence polarization assay for fetal lung maturity. The procedure requires 0.5 mL of amniotic fluid and a standard clinical laboratory fluorescence polarimeter (TDx Analyzer, Abbott Laboratories). We measured the L/S ratios for 93 freshly collected amniotic fluids, uncontaminated with blood or meconium, collected within three days of delivery. The fluids were stored frozen for eight to 32 months, then thawed and assayed for net fluorescence polarization. Fourteen of the infants developed respiratory distress syndrome; five, transient tachypnea of the newborn; and 74, no respiratory distress. The polarization assay and lecithin/sphingomyelin ratio had equivalent receiver operating characteristic curves, indicating no difference in their clinical performance. Although a prospective study with fresh amniotic fluid specimens will be necessary to establish a definitive reference range, the present study shows that this assay can be used to rapidly predict fetal lung maturity.
E R Ashwood; J F Tait; C A Foerder; R W Franklin; T J Benedetti
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  32     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-03-18     Completed Date:  1986-03-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  260-4     Citation Subset:  IM    
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MeSH Terms
Amniotic Fluid / analysis*
Fetal Organ Maturity*
Fluorescence Polarization / methods
Gestational Age
Infant, Newborn
Lung / embryology*
Phosphatidylcholines / analysis*
Prenatal Diagnosis
Respiration Disorders / diagnosis
Respiratory Distress Syndrome, Newborn / diagnosis
Retrospective Studies
Specimen Handling
Sphingomyelins / analysis*
Reg. No./Substance:
0/Phosphatidylcholines; 0/Sphingomyelins

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