Document Detail

Improved extracellular phytase activity in Saccharomyces cerevisiae by modifications in the PHO system.
MedLine Citation:
PMID:  16476497     Owner:  NLM     Status:  MEDLINE    
Myo-inositol hexaphosphate (IP6, phytate) is a potent anti-nutritional compound occurring in many plant-based staple foods, limiting the bioavailability of important nutrients such as iron and zinc. The objective of the present study was to investigate different strategies to achieve high and constitutive extracellular IP6 degradation by Baker's yeast, Saccharomyces cerevisiae. By deleting either of the genes PHO80 and PHO85, encoding negative regulators of the transcription of the repressible acid phosphatases (rAPs), the IP6 degradation became constitutive, and the biomass specific IP6 degradation was increased manyfold. In addition, the genes encoding the transcriptional activator Pho4p and the major rAP Pho5p were overexpressed in both a wild-type and a pho80delta strain, yielding an additional increase in IP6 degradation. It has previously been proved possible to increase human iron bioavailability by degradation of IP6 using microbial phytase. A high-phytase S. cerevisiae strain, without the use of any heterologous DNA, may be a suitable organism for the production of food-grade phytase and for the direct use in food production.
Jenny Veide; Thomas Andlid
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-14
Journal Detail:
Title:  International journal of food microbiology     Volume:  108     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-03     Completed Date:  2006-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  60-7     Citation Subset:  IM    
Chalmers University of Technology, Department of Chemical and Biological Engineering/Food Science, Box 5401, SE-402 29 Göteborg, Sweden.
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MeSH Terms
6-Phytase / metabolism*
Biological Availability
Food Microbiology*
Gene Deletion
Iron, Dietary / pharmacokinetics
Phytic Acid / metabolism*
Saccharomyces cerevisiae / enzymology*,  genetics*,  metabolism
Zinc / pharmacokinetics
Reg. No./Substance:
0/Iron, Dietary; 7440-66-6/Zinc; 83-86-3/Phytic Acid; EC

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