| Improved ethanol precipitation of DNA. | |
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MedLine Citation:
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PMID: 20336673 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this Short Communication, a shorter version of the standard DNA ethanol precipitation and purification protocol is described. It uses a mixture of 70% ethanol, 75 mM ammonium acetate and different concentrations of different carriers to perform DNA precipitation and washing in only one step. |
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Authors:
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Rosa Fregel; Ana González; Vicente M Cabrera |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 31 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-27 Completed Date: 2010-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 1350-2 Citation Subset: IM |
Affiliation:
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Area de Genética, Departamento de Parasitología, Ecología y Genética, Facultad de Biología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez, Tenerife, Spain. rfregel@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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chemistry Acrylamide / chemistry Chemical Precipitation DNA / chemistry, isolation & purification* DNA, Mitochondrial Electrophoresis, Agar Gel / methods* Ethanol / chemistry* Glycogen / chemistry Humans Polymerase Chain Reaction Temperature |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/DNA, Mitochondrial; 631-61-8/ammonium acetate; 64-17-5/Ethanol; 79-06-1/Acrylamide; 9005-79-2/Glycogen; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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