Document Detail

Improved efficiency of hybridoma ascites production by intrasplenic inoculation in mice.
MedLine Citation:
PMID:  6339777     Owner:  NLM     Status:  MEDLINE    
Intrasplenic inoculation of small numbers (10(4)) of antibody-producing hybridoma cells into unprimed BALB/c mice resulted in high-titered ascites. By intrasplenic injection, the low number of cells needed to produce ascites was 2-3 logs fewer than that required by ip inoculation. Hybridoma cells suspended in the ascitic fluid could be reestablished in vitro. Furthermore, cells obtained directly from individual, cloned colonies or from previously frozen samples also resulted in high-titered ascites, if the cells were inoculated intrasplenically. This method reduces time and expense required to grow large numbers of hybridoma cells for ip-derived ascites production and allows ready recovery of frequently unstable frozen-thawed cells.
P L Witte; R Ber
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the National Cancer Institute     Volume:  70     ISSN:  0027-8874     ISO Abbreviation:  J. Natl. Cancer Inst.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7503089     Medline TA:  J Natl Cancer Inst     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  575-7     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / biosynthesis
Ascitic Fluid / immunology,  metabolism*
Cytological Techniques
Hybridomas / metabolism,  transplantation*
Immunologic Techniques
Mice, Inbred BALB C
Peritoneum / metabolism
Spleen / metabolism*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal

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