| Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer. | |
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MedLine Citation:
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PMID: 17071774 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Somatic cell nuclear transfer (SCNT) has been used for the cloning of various mammals. However, the rates of successful, healthy birth are generally poor. To improve cloning efficiency, we report the utilization of an 'autologous SCNT' cloning technique in which the somatic nucleus of a female bovine donor is transferred to its own enucleated oocyte recovered by ovum pick up, in contrast to the routine 'allogeneic SCNT' procedure using oocytes from unrelated females. Our results showed that embryos derived from autologous SCNThave significantly higher developmental competence than those derived from allogeneic SCNT, especiallyat the eight-cell (60 vs 44%), morula (45 vs 36%), and blastocyst (38 vs 23%) stages. The pregnancy and birth rates were also higher for the autologous (39 and 23%), compared to the allogeneic (22 and 6%) SCNT groups. Genome-wide histone3-lysine9 methylation profiles reveal that autologous SCNTembryos have less epigenetic defects than the allogeneic SCNTembryos. This study indicates that autologous SCNT can improve the efficiency of bovine cloning with less reprogramming deficiency. |
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Authors:
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Xiao-Yu Yang; Hua Li; Qing-Wen Ma; Jing-Bin Yan; Jiang-Guo Zhao; Hua-Wei Li; Hai-Qing Shen; Hai-Feng Liu; Ying Huang; Shu-Zhen Huang; Yi-Tao Zeng; Fanyi Zeng |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: 132 ISSN: 1470-1626 ISO Abbreviation: Reproduction Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-30 Completed Date: 2007-06-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
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Languages: eng Pagination: 733-9 Citation Subset: IM |
Affiliation:
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Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, 24/1400 West Beijing Road, Shanghai 200040, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle* Cloning, Organism / methods* DNA / analysis DNA Methylation Embryo Culture Techniques Embryonic Development Female Fluorescent Antibody Technique, Indirect Male Nuclear Transfer Techniques* Pregnancy Pregnancy Outcome Sequence Analysis, DNA Transplantation, Autologous Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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