Document Detail


Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer.
MedLine Citation:
PMID:  17071774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Somatic cell nuclear transfer (SCNT) has been used for the cloning of various mammals. However, the rates of successful, healthy birth are generally poor. To improve cloning efficiency, we report the utilization of an 'autologous SCNT' cloning technique in which the somatic nucleus of a female bovine donor is transferred to its own enucleated oocyte recovered by ovum pick up, in contrast to the routine 'allogeneic SCNT' procedure using oocytes from unrelated females. Our results showed that embryos derived from autologous SCNThave significantly higher developmental competence than those derived from allogeneic SCNT, especiallyat the eight-cell (60 vs 44%), morula (45 vs 36%), and blastocyst (38 vs 23%) stages. The pregnancy and birth rates were also higher for the autologous (39 and 23%), compared to the allogeneic (22 and 6%) SCNT groups. Genome-wide histone3-lysine9 methylation profiles reveal that autologous SCNTembryos have less epigenetic defects than the allogeneic SCNTembryos. This study indicates that autologous SCNT can improve the efficiency of bovine cloning with less reprogramming deficiency.
Authors:
Xiao-Yu Yang; Hua Li; Qing-Wen Ma; Jing-Bin Yan; Jiang-Guo Zhao; Hua-Wei Li; Hai-Qing Shen; Hai-Feng Liu; Ying Huang; Shu-Zhen Huang; Yi-Tao Zeng; Fanyi Zeng
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  132     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-30     Completed Date:  2007-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  733-9     Citation Subset:  IM    
Affiliation:
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, 24/1400 West Beijing Road, Shanghai 200040, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle*
Cloning, Organism / methods*
DNA / analysis
DNA Methylation
Embryo Culture Techniques
Embryonic Development
Female
Fluorescent Antibody Technique, Indirect
Male
Nuclear Transfer Techniques*
Pregnancy
Pregnancy Outcome
Sequence Analysis, DNA
Transplantation, Autologous
Transplantation, Homologous
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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