Document Detail


Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments.
MedLine Citation:
PMID:  11097948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a single freeze-thaw treatment. The number of viable cells was obtained by subtracting the number of remaining cells after the qDVC procedure from the total cell number before the qDVC incubation. This improved procedure should provide useful information about the metabolic potential of natural bacterial communities.
Authors:
D Yokomaku; N Yamaguchi; M Nasu
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  66     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-14     Completed Date:  2001-02-15     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5544-8     Citation Subset:  IM    
Affiliation:
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / cytology,  isolation & purification*,  metabolism
Colony Count, Microbial / methods*
Escherichia coli / cytology,  isolation & purification,  metabolism
Fresh Water / microbiology*
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