| Improved determination of small amounts of free hydroxyproline in biological fluids. | |
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MedLine Citation:
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PMID: 8070084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fast, reliable, and sensitive (< 20 pmol) method for the quantification of 4-hydroxyproline is described. It ensures good separation of imino acid peaks, eliminates interference by primary amino acids, and guarantees full (96-105%) recovery of hydroxyproline (HYP). Interfering primary amino acids are derivatized by o-phthaldialdehyde and removed from the sample by use of a discardable C18 column. HYP is measured photometrically at 254 nm after a second derivatization with phenylisothiocyanate and isocratic separation on a reversed-phase HPLC column. |
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Authors:
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M Lange; M Mályusz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry Volume: 40 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1994 Sep |
Date Detail:
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Created Date: 1994-09-29 Completed Date: 1994-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1735-8 Citation Subset: IM |
Affiliation:
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Physiologisches Institut der Universität Kiel, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Humans Hydroxyproline / analysis*, blood, urine Isothiocyanates Specimen Handling Spectrophotometry, Ultraviolet Thiocyanates |
| Chemical | |
Reg. No./Substance:
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0/Isothiocyanates; 0/Thiocyanates; 103-72-0/phenylisothiocyanate; 51-35-4/Hydroxyproline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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