Document Detail

Improved degradation of organophosphorus nerve agents and p-nitrophenol by Pseudomonas putida JS444 with surface-expressed organophosphorus hydrolase.
MedLine Citation:
PMID:  15932242     Owner:  NLM     Status:  MEDLINE    
Pseudomonas putida JS444, isolated from p-nitrophenol (PNP) contaminated waste sites, was genetically engineered to simultaneously degrade organophosphorus pesticides (OP) and PNP. A surface anchor system derived from the ice-nucleation protein (INP) from Pseudomonas syringae was used to target the organophosphorus hydrolase (OPH) onto the surface of Pseudomonas putida JS444, reducing the potential substrate uptake limitation. Engineered cells were capable of targeting OPH onto the cell surface as demonstrated by western blotting, cell fractionation, and immunofluorescence microscopy. The engineered P. putida JS444 degraded organophosphates as well as PNP rapidly without instability problems associated with the engineered Moraxella sp. The initial hydrolysis rate was 7.90, 3.54, and 1.53 micromol/h/mg dry weight for paraoxon, parathion, and methyl parathion, respectively. The excellent stability in combination with the rapid degradation rate for organophosphates and PNP make this engineered strain an ideal biocatalyst for complete mineralization of organophosphates.
Yu Lei; Ashok Mulchandani; Wilfred Chen
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biotechnology progress     Volume:  21     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-06-03     Completed Date:  2005-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  678-81     Citation Subset:  IM    
Department of Chemical and Environmental Engineeirng, University of California, Riverside, California 92521, USA.
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MeSH Terms
Aryldialkylphosphatase / genetics,  metabolism*
Biodegradation, Environmental
Chemical Warfare Agents / pharmacokinetics
Environmental Pollution / prevention & control
Genetic Enhancement / methods*
Insecticides / pharmacokinetics*
Membrane Proteins / genetics,  metabolism*
Nitrophenols / pharmacokinetics*
Organophosphorus Compounds / pharmacokinetics*
Protein Engineering / methods
Pseudomonas putida / metabolism*
Recombinant Proteins / metabolism
Reg. No./Substance:
0/Chemical Warfare Agents; 0/Insecticides; 0/Membrane Proteins; 0/Nitrophenols; 0/Organophosphorus Compounds; 0/Recombinant Proteins; 100-02-7/4-nitrophenol; EC

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