Document Detail

Improved cold preservation of kidney tubular cells by means of adding bioflavonoids to organ preservation solutions.
MedLine Citation:
PMID:  16436967     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Cold ischemia and reperfusion during renal transplantation result in release of reactive oxygen species. The aim of this study is to examine whether cold storage induced cell injury can be ameliorated by adding flavonoids directly to preservation solutions. METHODS: Cultured renal tubular epithelial cells (LLC-PK1) were stored in University of Wisconsin (UW) or Euro-Collins (EC) solution at 4 degrees C for 20 hours. Preservation solutions were supplemented with various flavonoids. After rewarming, structural and metabolic cell integrity was measured by lactate dehydrogenase (LDH) release and MTT-test, and lipid peroxidation was assessed from generation of thiobarbituric acid-reactive substances (TBARS). RESULTS: Twenty hours of cold storage resulted in a substantial loss of cell viability in both preservation solutions (in EC: LDH release 92.4+/-2.7%; MTT-test 0.5+/-0.7%). Addition of luteolin, quercetin, kempferol, fisetin, myricetin, morin, catechin, and silibinin significantly reduced cell injury (for luteolin in EC: LDH release 2.4+/-1.6%; MTT-test 110.3+/-10.4%, P<0.01; TBARS-production (related to cold stored control cells) 8.9+/-2.6%). No cytoprotection was found for apigenin, naringenin, and rutin. Protective potency of flavonoids depends on number of hydroxyl-substituents and lipophilicity of the diphenylpyran compounds. CONCLUSION: Cold storage induced injury of renal tubular cells was substantially ameliorated by adding selected flavonoids directly to preservation solutions.
Thurid Ahlenstiel; Gunther Burkhardt; Hans Köhler; Martin K Kuhlmann
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Transplantation     Volume:  81     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-26     Completed Date:  2006-02-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  231-9     Citation Subset:  IM    
Department of Medicine, Division of Nephrology and Hypertension, University Hospital of Saarland, Homburg/Saar, Germany.
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MeSH Terms
Cell Survival / drug effects
Cold Temperature
Cryoprotective Agents* / administration & dosage
Flavonoids* / administration & dosage,  chemistry
Hypertonic Solutions
Kidney Transplantation
Kidney Tubules, Proximal / cytology*,  metabolism
L-Lactate Dehydrogenase / metabolism
LLC-PK1 Cells
Lipid Peroxidation / drug effects
Luteolin / administration & dosage
Molecular Structure
Organ Preservation / methods*
Organ Preservation Solutions*
Quercetin / administration & dosage
Reperfusion Injury / prevention & control
Reg. No./Substance:
0/Cryoprotective Agents; 0/Euro-Collins' solution; 0/Flavonoids; 0/Hypertonic Solutions; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 117-39-5/Quercetin; 315-30-0/Allopurinol; 491-70-3/Luteolin; 512-69-6/Raffinose; 58-61-7/Adenosine; 70-18-8/Glutathione; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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