Document Detail


Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells.
MedLine Citation:
PMID:  4322279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved tissue culture basal medium, CMRL-1969, supplemented with serum, has been evaluated by measuring the growth responses of primary cultures of trypsin-dispersed monkey kidney cells (PMKC) and of an established culture of a human diploid cell strain (HDCS). Medium H597, an early modification of medium 199 which has been used successfully in the preparation of poliomyelitis vaccine for 15 years, was used for comparison. In addition, parallel testing was done with Basal Medium Eagle (BME) widely used for the growth of HDCS. The improvements in basal medium CMRL-1969 are attributed to changes in amino acid concentrations, in vitamin composition, and, in particular, to enhanced buffering capacity. The latter has been achieved by the use of free-base amino acids and by increasing the dibasic sodium phosphate. The new medium has already been used to advantage for the production of polioviruses in PMKC where equivalent titers were obtained from cultures initiated with 70% of the number of cells required with earlier media. The population-doubling time was reduced in this system. Also, with small inocula of HDCS, the time required to obtain maximum cell yield was shorter with CMRL-1969 than with BME. Both media were supplemented with 10% calf serum. Maximum cell yields after repeated subcultivation in the new basal medium were greatly increased and the stability of the strain, as shown by chromosomal analysis, was not affected. Basal medium CMRL-1969 can be prepared easily in liquid or powdered form.
Authors:
G M Healy; S Teleki; A von Seefried; M J Walton; H G Macmorine
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  21     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1971 Jan 
Date Detail:
Created Date:  1971-03-12     Completed Date:  1971-03-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids
Animals
Buffers
Culture Media*
Culture Techniques*
Diploidy*
Galactose
Glucose
Haplorhini
Humans
Poliovirus / growth & development
Serum Albumin, Bovine
Trypsin
Vitamins
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Buffers; 0/Culture Media; 0/Serum Albumin, Bovine; 0/Vitamins; 26566-61-0/Galactose; 50-99-7/Glucose; EC 3.4.21.4/Trypsin
Comments/Corrections

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