| Improved biochemical preservation of heart slices during cold storage. | |
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MedLine Citation:
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PMID: 12678509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Development of myocardial preservation solutions requires the use of whole organ models which are animal and labor intensive. These models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. We hypothesized that myocardial slices could be used to assess preservation of biochemical function during cold storage. MATERIALS AND METHODS: Whole rat hearts were precision cut into slices with a thickness of 200 microm and preserved at 4 degrees C in one of the following solutions: Columbia University (CU), University of Wisconsin (UW), D5 0.2% normal saline with 20 meq/l KCL (QNS), normal saline (NS), or a novel cardiac preservation solution (NPS) developed using this model. Myocardial biochemical function was assessed by ATP content (etamoles ATP/mg wet weight) and capacity for protein synthesis (counts per minute (cpm)/mg protein) immediately following slicing (0 hours), and at 6, 12, 18, and 24 hours of cold storage. Six slices were assayed at each time point for each solution. The data were analyzed using analysis of variance and are presented as the mean +/- standard deviation. RESULTS: ATP content was higher in the heart slices stored in the NPS compared to all other solutions at 6, 12, 18 and 24 hours of cold storage (p < 0.05). Capacity for protein synthesis was higher in the heart slices stored in the NPS compared to all other solutions at 6, 12, and 18 hours of cold storage (p < 0.05). CONCLUSIONS This myocardial slice model allows the rapid and efficient screening of cardiac preservation solutions and their components using quantifiable biochemical endpoints. Using this model, we have developed a novel preservation solution which improves the biochemical function of myocardial slices during cold storage. |
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Authors:
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D A Bull; B B Reid; R C Connors; A Albanil; J C Stringham; S V Karwande |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of surgical investigation Volume: 2 ISSN: 1028-5229 ISO Abbreviation: Int. J. Surg. Investig. Publication Date: 2000 |
Date Detail:
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Created Date: 2003-04-07 Completed Date: 2003-07-29 Revised Date: 2006-07-25 |
Medline Journal Info:
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Nlm Unique ID: 100965774 Medline TA: Int J Surg Investig Country: England |
Other Details:
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Languages: eng Pagination: 117-23 Citation Subset: IM |
Affiliation:
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Division of Cardiothoracic Surgery, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA. david.bull@hsc.utah.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism* Animals Cryopreservation* Models, Animal Myocardium / metabolism* Organ Preservation Solutions* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Organ Preservation Solutions; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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