Document Detail

Improved biochemical preservation of heart slices during cold storage.
MedLine Citation:
PMID:  12678509     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Development of myocardial preservation solutions requires the use of whole organ models which are animal and labor intensive. These models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. We hypothesized that myocardial slices could be used to assess preservation of biochemical function during cold storage. MATERIALS AND METHODS: Whole rat hearts were precision cut into slices with a thickness of 200 microm and preserved at 4 degrees C in one of the following solutions: Columbia University (CU), University of Wisconsin (UW), D5 0.2% normal saline with 20 meq/l KCL (QNS), normal saline (NS), or a novel cardiac preservation solution (NPS) developed using this model. Myocardial biochemical function was assessed by ATP content (etamoles ATP/mg wet weight) and capacity for protein synthesis (counts per minute (cpm)/mg protein) immediately following slicing (0 hours), and at 6, 12, 18, and 24 hours of cold storage. Six slices were assayed at each time point for each solution. The data were analyzed using analysis of variance and are presented as the mean +/- standard deviation. RESULTS: ATP content was higher in the heart slices stored in the NPS compared to all other solutions at 6, 12, 18 and 24 hours of cold storage (p < 0.05). Capacity for protein synthesis was higher in the heart slices stored in the NPS compared to all other solutions at 6, 12, and 18 hours of cold storage (p < 0.05). CONCLUSIONS This myocardial slice model allows the rapid and efficient screening of cardiac preservation solutions and their components using quantifiable biochemical endpoints. Using this model, we have developed a novel preservation solution which improves the biochemical function of myocardial slices during cold storage.
D A Bull; B B Reid; R C Connors; A Albanil; J C Stringham; S V Karwande
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of surgical investigation     Volume:  2     ISSN:  1028-5229     ISO Abbreviation:  Int. J. Surg. Investig.     Publication Date:  2000  
Date Detail:
Created Date:  2003-04-07     Completed Date:  2003-07-29     Revised Date:  2006-07-25    
Medline Journal Info:
Nlm Unique ID:  100965774     Medline TA:  Int J Surg Investig     Country:  England    
Other Details:
Languages:  eng     Pagination:  117-23     Citation Subset:  IM    
Division of Cardiothoracic Surgery, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA.
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MeSH Terms
Adenosine Triphosphate / metabolism*
Models, Animal
Myocardium / metabolism*
Organ Preservation Solutions*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Organ Preservation Solutions; 56-65-5/Adenosine Triphosphate

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