Document Detail


Improved autoradiographic procedure for cytogenetics: inverted coverslips and double illumination.
MedLine Citation:
PMID:  6171391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The method described here has the advantage of presenting a clear image of both chromosomes and silver grains. Chromosomes are stained through the emulsion with Hoechst 33258. As two different sources of light are employed-epi-ultraviolet illumination and transmitted visible light-a separate photographic record of each optical plane can be obtained of chromosomes, silver grains, and chromosomes and grains together.
Authors:
M Stoll; H Cardoso
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  31     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1981  
Date Detail:
Created Date:  1982-02-25     Completed Date:  1982-02-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  101-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography / methods*
Bisbenzimidazole
Chromosomes / metabolism,  ultrastructure*
Cytogenetics*
DNA Replication
Lighting
Male
Methods
Orthoptera / ultrastructure*
RNA / biosynthesis
Staining and Labeling
Ultraviolet Rays
Chemical
Reg. No./Substance:
23491-44-3/Bisbenzimidazole; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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