Document Detail

Improved assay for monocyte chemotaxis using frozen stored responder cells.
MedLine Citation:
PMID:  833430     Owner:  NLM     Status:  MEDLINE    
Frozen stored human mononuclear cells were compared with non-frozen cells as responder cells in the monocyte chemotaxis assay. Frozen stored cells were found to inhibit lower background migration, resulting in greater experimental-to-control ratios. The use of frozen cells also greatly improved the speed, standardization of conditions and day-to-day reproducibility of the assay procedure. Frozen cells were at least as sensitive as fresh cells for detecting low chemotactic factor concentrations and were superior for detecting differences in concentration.
D A Dean; D M Strong
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  14     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1977  
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-72     Citation Subset:  IM    
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MeSH Terms
Blood Preservation*
Cell Membrane
Chemotaxis, Leukocyte*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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