Document Detail


Improved assay for monocyte chemotaxis using frozen stored responder cells.
MedLine Citation:
PMID:  833430     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Frozen stored human mononuclear cells were compared with non-frozen cells as responder cells in the monocyte chemotaxis assay. Frozen stored cells were found to inhibit lower background migration, resulting in greater experimental-to-control ratios. The use of frozen cells also greatly improved the speed, standardization of conditions and day-to-day reproducibility of the assay procedure. Frozen cells were at least as sensitive as fresh cells for detecting low chemotactic factor concentrations and were superior for detecting differences in concentration.
Authors:
D A Dean; D M Strong
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  14     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1977  
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Preservation*
Cell Membrane
Chemotaxis, Leukocyte*
Freezing
Humans
Monocytes*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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