Document Detail

Improved assay method for phospholipase C.
MedLine Citation:
PMID:  4962285     Owner:  NLM     Status:  MEDLINE    
A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.
S Kurioka; P V Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  15     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1967 May 
Date Detail:
Created Date:  1967-10-14     Completed Date:  1967-10-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  551-5     Citation Subset:  IM    
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MeSH Terms
Alkaline Phosphatase / metabolism
Bacillus cereus / enzymology
Clostridium / enzymology
Detergents / pharmacology
Escherichia coli / enzymology
Phosphates / analysis
Phosphatidylcholines / metabolism
Phospholipases / analysis*
Pseudomonas aeruginosa / enzymology
Reg. No./Substance:
0/Detergents; 0/Phosphates; 0/Phosphatidylcholines; EC 3.1.-/Phospholipases; EC Phosphatase

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