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Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification.
MedLine Citation:
PMID:  23182040     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20g/L of glucose to produce lactic acid at a yield of 95% in 1L MRS medium at pH 3.8 within 36h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms.
Authors:
Lidan Ye; Hua Zhao; Zhi Li; Jin Chuan Wu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-22
Journal Detail:
Title:  Bioresource technology     Volume:  -     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A(∗)STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Singapore; Department of Chemical and Biomolecular Engineering, Faculty of Engineering, National University of Singapore, Blk E5, 4 Engineering Drive 4, Singapore 117576, Singapore.
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