| Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification. | |
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MedLine Citation:
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PMID: 23182040 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20g/L of glucose to produce lactic acid at a yield of 95% in 1L MRS medium at pH 3.8 within 36h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms. |
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Authors:
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Lidan Ye; Hua Zhao; Zhi Li; Jin Chuan Wu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-22 |
Journal Detail:
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Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: Bioresour. Technol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-11-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A(∗)STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Singapore; Department of Chemical and Biomolecular Engineering, Faculty of Engineering, National University of Singapore, Blk E5, 4 Engineering Drive 4, Singapore 117576, Singapore. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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