Document Detail

Improved Production of Long-Chain Fatty Acid in Escherichia coli by an Engineering Elongation Cycle During Fatty Acid Synthesis (FAS) Through Genetic Manipulation.
MedLine Citation:
PMID:  22580319     Owner:  NLM     Status:  In-Data-Review    
The microbial biosynthesis of fatty acid of lipid metabolism, which can be used as precursors for the production of fuels of chemicals from renewable carbon sources, has attracted significant attention in recent years. The regulation of fatty acid biosynthesis pathways has been mainly studied in a model prokaryote, Escherichia coli. During the recent period, global regulation of fatty acid metabolic pathways has been demonstrated in another model prokaryote, Bacillus subtilis, as well as in Streptococcus pneumonia. The goal of this study was to increase the production of long-chain fatty acids by developing recombinant E. coli strains that were improved by an elongation cycle of fatty acid synthesis (FAS). The fabB, fabG, fabZ, and fabI genes, all homologous of E. coli, were induced to improve the enzymatic activities for the purpose of overexpressing components of the elongation cycle in the FAS pathway through metabolic engineering. The beta-oxoacyl-ACP synthase enzyme catalyzed the addition of acyl-ACP to malonyl-ACP to generate beta- oxoacyl-ACP. The enzyme encoded by the fabG gene converted beta-oxoacyl-ACP to beta-hydroxyacyl-ACP, the fabZ catalyzed the dehydration of beta-3-hydroxyacyl-ACP to trans-2-acyl-ACP, and the fabI gene converted trans-2- acyl-ACP to acyl-ACP for long-chain fatty acids. In vivo productivity of total lipids and fatty acids was analyzed to confirm the changes and effects of the inserted genes in E. coli. As a result, lipid was increased 2.16-fold higher and hexadecanoic acid was produced 2.77-fold higher in E. coli JES1030, one of the developed recombinants through this study, than those from the wild-type E. coli.
Eunyoung Jeon; Sunhee Lee; Seunghan Lee; Sung Ok Han; Yeo Joon Yoon; Jinwon Lee
Related Documents :
20615689 - Production potential of chlorella zofingienesis as a feedstock for biodiesel.
20379849 - Alteration of the phospho- or neutral lipid content and fatty acid composition in liste...
18211819 - Comparison of rat epidermal keratinocyte organotypic culture (roc) with intact human sk...
2349579 - Identification of okadaic acid from a caribbean dinoflagellate, prorocentrum concavum.
19696439 - Demonstrated and inferred metabolism associated with cytosolic lipid droplets.
10733669 - Barrier characteristics of different human skin types investigated with x-ray diffracti...
18590529 - A rapid and robust method for simultaneously measuring changes in the phytohormones aba...
16968879 - Sethoxydim affects lipid synthesis and acetyl-coa carboxylase activity in soybean.
3989789 - Cyclic changes in phospholipid content and composition in human endometrium during the ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microbiology and biotechnology     Volume:  22     ISSN:  1738-8872     ISO Abbreviation:  J. Microbiol. Biotechnol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-05-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431852     Medline TA:  J Microbiol Biotechnol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  990-9     Citation Subset:  IM    
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Growth Properties and Cholesterol Removal Ability of Electroporated Lactobacillus acidophilus BT 108...
Next Document:  Production of Platinum Nanoparticles and Nanoaggregates Using Neurospora crassa.