| Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome. | |
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MedLine Citation:
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PMID: 20692400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of the pancreas by implementing a vascular flush and ductal preservation. METHOD: Pancreata procured from adult Landrace pigs were divided into 3 different surgical protocols: Pancreatectomy utilizing only surface cooling (group 1; n = 24); surface cooling and ductal injection with cold preservation solution before pancreatectomy (group 2; n = 12); or surface cooling, ductal injection, and an approach by selectively flushing through the celiac trunk and the superior mesenteric artery (group 3; n = 14). We assessed the islet isolation results and quality using in vitro and in vivo assays. RESULTS: Significantly higher overall yield and islet yield per gram pancreas were obtained from group 3 pigs compared with the other groups. Measurements of islet viability after 7 days of culture, as assessed by oxygen consumption rate per DNA, showed that group 3 islets displayed the highest values. Sustained normoglycemia was observed in diabetic nude mice transplanted with 2000 islet equivalents from all 3 groups. DISCUSSION: This study demonstrated that an advanced pancreas procurement technique including ductal preservation and selective arterial flush with cold preservation solution provided significant improvements in porcine islet isolation outcomes. |
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Authors:
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T Anazawa; A N Balamurugan; K K Papas; E S Avgoustiniatos; J Ferrer; S Matsumoto; D E R Sutherland; B J Hering |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation proceedings Volume: 42 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2010 Jul-Aug |
Date Detail:
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Created Date: 2010-08-09 Completed Date: 2011-01-14 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 2032-5 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota 55455, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Weight Cell Separation / methods Cell Survival Death Islets of Langerhans / cytology*, physiology Islets of Langerhans Transplantation / physiology Mice Mice, Nude Organ Preservation Solutions Organ Size Oxygen Consumption Pancreas / anatomy & histology*, blood supply, cytology Pancreatectomy / methods Swine Transplantation, Heterologous |
| Grant Support | |
ID/Acronym/Agency:
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U19 AI067151/AI/NIAID NIH HHS; U19 AI067151-04/AI/NIAID NIH HHS; U19 AI067151-05/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Organ Preservation Solutions |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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