Document Detail


Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome.
MedLine Citation:
PMID:  20692400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of the pancreas by implementing a vascular flush and ductal preservation.
METHOD: Pancreata procured from adult Landrace pigs were divided into 3 different surgical protocols: Pancreatectomy utilizing only surface cooling (group 1; n = 24); surface cooling and ductal injection with cold preservation solution before pancreatectomy (group 2; n = 12); or surface cooling, ductal injection, and an approach by selectively flushing through the celiac trunk and the superior mesenteric artery (group 3; n = 14). We assessed the islet isolation results and quality using in vitro and in vivo assays.
RESULTS: Significantly higher overall yield and islet yield per gram pancreas were obtained from group 3 pigs compared with the other groups. Measurements of islet viability after 7 days of culture, as assessed by oxygen consumption rate per DNA, showed that group 3 islets displayed the highest values. Sustained normoglycemia was observed in diabetic nude mice transplanted with 2000 islet equivalents from all 3 groups.
DISCUSSION: This study demonstrated that an advanced pancreas procurement technique including ductal preservation and selective arterial flush with cold preservation solution provided significant improvements in porcine islet isolation outcomes.
Authors:
T Anazawa; A N Balamurugan; K K Papas; E S Avgoustiniatos; J Ferrer; S Matsumoto; D E R Sutherland; B J Hering
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation proceedings     Volume:  42     ISSN:  1873-2623     ISO Abbreviation:  Transplant. Proc.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-08-09     Completed Date:  2011-01-14     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2032-5     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota 55455, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight
Cell Separation / methods
Cell Survival
Death
Islets of Langerhans / cytology*,  physiology
Islets of Langerhans Transplantation / physiology
Mice
Mice, Nude
Organ Preservation Solutions
Organ Size
Oxygen Consumption
Pancreas / anatomy & histology*,  blood supply,  cytology
Pancreatectomy / methods
Swine
Transplantation, Heterologous
Grant Support
ID/Acronym/Agency:
U19 AI067151/AI/NIAID NIH HHS; U19 AI067151/AI/NIAID NIH HHS; U19 AI067151-04/AI/NIAID NIH HHS; U19 AI067151-05/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Organ Preservation Solutions
Comments/Corrections

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