| An improved method for determining medium- and long-chain FAMEs using gas chromatography. | |
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MedLine Citation:
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PMID: 20082149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The existing protocols for analyzing fatty acid methyl esters (FAMEs) using a one-step acetyl chloride (AC) catalyzed transesterification and extraction procedure cannot accurately determine the medium- and long-chain fatty acids simultaneously in clinical (enteral, parenteral) formulations. For example: (1) addition of AC at room temperature generates an exothermic reaction that often results in loss of sample and possible injury to the analyst; (2) certain polyunsaturated fatty acids (PUFAs) are less stable at elevated temperatures during the transesterification and contribute to the over-estimation of the C16:0 and C18:1 fatty acids; and (3) the flame-ionization detector (FID) response varies depending on the carbon chain length of the fatty acids, that consequently impacts the underestimation of medium-chain fatty acid (C6-C10) recoveries. To overcome these deficiencies and accurately determine FAMEs, we have developed an improved one-step transesterification method that employs the addition of AC in tubes kept on a dry ice bath, the transesterification at room temperature, and the data analysis using relative response factors. Using this modified protocol, we determined the fatty acid composition of lipid emulsions (Omegaven and Lipidem) on a Shimadzu GC2010 gas chromatography (GC) system using a capillary GC column (Zebron ZB-WAX plus, 30 m, 0.25 mm ID, 0.25 microm). Our data suggest that the improved method can be easily used to accurately determine fatty acids (C6-C24) in functional foods and lipid emulsions. |
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Authors:
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Zhidong Xu; Kevin Harvey; Thomas Pavlina; Guy Dutot; Gary Zaloga; Rafat Siddiqui |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 45 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-19 Completed Date: 2010-04-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 199-208 Citation Subset: IM |
Affiliation:
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Methodist Research Institute, Clarian Health, Indiana University School of Medicine, Indianapolis, IN 46202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gas
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methods* Esterification Esters / analysis* Fatty Acids / analysis* Flame Ionization |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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