Document Detail


An improved measurement of isotopic ratios by high resolution mass spectrometry.
MedLine Citation:
PMID:  23283729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The study of protein kinetics requires an accurate measurement of isotopic ratios of peptides. Although Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometers yield accurate mass measurements of analytes, the isotopologue ratios are consistently lower than predicted. Recently, we demonstrated that the magnitude of the spectral error in the FT-ICR mass spectrometer is proportional to the scan duration of ions. Here, we present a novel isotopic ratio extrapolation (IRE) method for obtaining accurate isotopic ratio measurements. Accuracy is achieved by performing scans with different duration and extrapolation of the data to the initial moment of the ion rotation; IRE minimizes the absolute isotopic ratio error to ≤1%. We demonstrate the application of IRE in protein turnover studies using (2)H(2)O-metabolic labeling. Overall, this technique allows accurate measurements of the isotopic ratios of proteolytic peptides, a critical step for enabling routine studies of proteome dynamics.
Authors:
Serguei Ilchenko; Stephen F Previs; Nadia Rachdaoui; Belinda Willard; Arthur J McCullough; Takhar Kasumov
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-03
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  24     ISSN:  1879-1123     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-11     Completed Date:  2013-07-22     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  309-12     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Albumins / analysis,  chemistry,  metabolism
Animals
Deuterium / analysis*,  metabolism
Isotope Labeling / methods*
Mass Spectrometry / methods*
Peptide Fragments / analysis,  chemistry,  metabolism
Peptides / analysis*,  chemistry,  metabolism
Proteome / analysis,  chemistry,  metabolism
Proteomics
Rats
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
5R21RR025346-02/RR/NCRR NIH HHS; R21 HL114407/HL/NHLBI NIH HHS; R21 RR025346/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Albumins; 0/Peptide Fragments; 0/Peptides; 0/Proteome; AR09D82C7G/Deuterium
Comments/Corrections

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