Document Detail


Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system.
MedLine Citation:
PMID:  21543564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current methods for identification of yeast from blood cultures may take several days after these microorganisms have been observed by Gram stain smears from positive blood cultures. We explored the use of a matrix-assisted laser desorption ionization (MALDI) Biotyper system in combination with Sepsityper specimen processing and Microflex analysis for improved detection and identification of yeast species directly from positive blood culture specimens demonstrating yeast-like organisms by Gram stain. The limit of detection of yeast species in blood culture medium was determined to be 5.9 × 10(5) CFU, with intra- and interstrain coefficients of variation of 1.8 to 3.6% and 2.9%, respectively. A total of 42 yeast-containing positive blood culture specimens were processed, and the identification results were compared to those obtained by routinely used phenotypic methods. Specimens with discrepant results between the Biotyper and phenotypic methods were identified on the basis of internal transcribed spacer region sequencing. The MALDI Biotyper system correctly identified the 42 specimens to species level, including 28 (66.7%) Candida albicans, 8 (19.0%) Candida parapsilosis, and 5 (11.9%) Candida tropicalis isolates and 1 (2.4%) Cryptococcus neoformans isolate. The entire procedure, from specimen extraction to final result reporting, can be completed within 1 h. Our data indicated that the Sepsityper specimen processing and Microflex analysis by the MALDI Biotyper system provide a rapid and reliable tool for yeast species identification directly from positive blood culture media.
Authors:
Yingjun Yan; Ying He; Thomas Maier; Criziel Quinn; Gongyi Shi; Haijing Li; Charles W Stratton; Markus Kostrzewa; Yi-Wei Tang
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2011-05-04
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  49     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-01     Completed Date:  2011-09-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2528-32     Citation Subset:  IM    
Affiliation:
Department of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232-5310, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/HQ214057
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MeSH Terms
Descriptor/Qualifier:
Blood / microbiology*
Clinical Laboratory Techniques / methods*
DNA, Fungal / chemistry,  genetics
DNA, Ribosomal Spacer / chemistry,  genetics
Humans
Molecular Sequence Data
Mycology / methods*
Sensitivity and Specificity
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Yeasts / classification*,  growth & development,  isolation & purification*
Grant Support
ID/Acronym/Agency:
UL1RR024975/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Ribosomal Spacer
Comments/Corrections

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