| Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system. | |
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MedLine Citation:
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PMID: 21543564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current methods for identification of yeast from blood cultures may take several days after these microorganisms have been observed by Gram stain smears from positive blood cultures. We explored the use of a matrix-assisted laser desorption ionization (MALDI) Biotyper system in combination with Sepsityper specimen processing and Microflex analysis for improved detection and identification of yeast species directly from positive blood culture specimens demonstrating yeast-like organisms by Gram stain. The limit of detection of yeast species in blood culture medium was determined to be 5.9 × 10(5) CFU, with intra- and interstrain coefficients of variation of 1.8 to 3.6% and 2.9%, respectively. A total of 42 yeast-containing positive blood culture specimens were processed, and the identification results were compared to those obtained by routinely used phenotypic methods. Specimens with discrepant results between the Biotyper and phenotypic methods were identified on the basis of internal transcribed spacer region sequencing. The MALDI Biotyper system correctly identified the 42 specimens to species level, including 28 (66.7%) Candida albicans, 8 (19.0%) Candida parapsilosis, and 5 (11.9%) Candida tropicalis isolates and 1 (2.4%) Cryptococcus neoformans isolate. The entire procedure, from specimen extraction to final result reporting, can be completed within 1 h. Our data indicated that the Sepsityper specimen processing and Microflex analysis by the MALDI Biotyper system provide a rapid and reliable tool for yeast species identification directly from positive blood culture media. |
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Authors:
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Yingjun Yan; Ying He; Thomas Maier; Criziel Quinn; Gongyi Shi; Haijing Li; Charles W Stratton; Markus Kostrzewa; Yi-Wei Tang |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2011-05-04 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 49 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-07-01 Completed Date: 2011-09-26 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2528-32 Citation Subset: IM |
Affiliation:
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Department of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232-5310, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/HQ214057 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood
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microbiology* Clinical Laboratory Techniques / methods* DNA, Fungal / chemistry, genetics DNA, Ribosomal Spacer / chemistry, genetics Humans Molecular Sequence Data Mycology / methods* Sensitivity and Specificity Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Yeasts / classification*, growth & development, isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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UL1RR024975/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal; 0/DNA, Ribosomal Spacer |
| Comments/Corrections | |
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