Document Detail


Improved Folate Extraction and Tracing Deconjugation Efficiency by Dual Label Isotope Dilution Assays in Foods.
MedLine Citation:
PMID:  22235748     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A dual label stable isotope dilution assay was developed to trace the deconjugation efficiency of polyglutamic folate vitamers converted to their monoglutamic analogues. For this purpose, [13C5]-pteroylheptaglutamate was synthesized and added during extraction of foods as a tracer isotopologue along with [2H4]-5-methyltetrahydrofolate, [2H4]-5-formyltetrahydrofolate, [2H4]-tetrahydrofolate, [2H4]-10-formylfolate and [2H4]-folic acid. The [2H4]-labelled folates were used as internal standards for the monoglutamates. Deconjugation converted the addition tracer [13C5]-pteroylheptaglutamate to the detection tracer [13C5]-folic acid, which was quantified along with unlabelled folic acid using [2H4]-folic acid as the internal standard. LC-MS/MS enabled the unequivocal differentiation of the three isotopologues. This tracing was used to optimize deconjugation efficiency, which was achieved by using 4-morpholineethanesulfonic acid buffer for extraction at pH 5.0 . The optimized assay revealed limits of detection for the folate vitamers ranging between 2.0 and 5.6 pmol per assay (equivalent to 2.2 - 6.6 µg/ 100 g dry mass), recoveries ranging between 98 and 105 % and relative standard deviations in inter-assay precision ranging between 2 and 6 %. The assay was applied to quantitate folates in spinach, beans, cheeses, bread, wheat germs, and yeast .
Authors:
Sabine Mönch; Michael Rychlik
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-11
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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