| Improved Folate Extraction and Tracing Deconjugation Efficiency by Dual Label Isotope Dilution Assays in Foods. | |
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MedLine Citation:
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PMID: 22235748 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A dual label stable isotope dilution assay was developed to trace the deconjugation efficiency of polyglutamic folate vitamers converted to their monoglutamic analogues. For this purpose, [13C5]-pteroylheptaglutamate was synthesized and added during extraction of foods as a tracer isotopologue along with [2H4]-5-methyltetrahydrofolate, [2H4]-5-formyltetrahydrofolate, [2H4]-tetrahydrofolate, [2H4]-10-formylfolate and [2H4]-folic acid. The [2H4]-labelled folates were used as internal standards for the monoglutamates. Deconjugation converted the addition tracer [13C5]-pteroylheptaglutamate to the detection tracer [13C5]-folic acid, which was quantified along with unlabelled folic acid using [2H4]-folic acid as the internal standard. LC-MS/MS enabled the unequivocal differentiation of the three isotopologues. This tracing was used to optimize deconjugation efficiency, which was achieved by using 4-morpholineethanesulfonic acid buffer for extraction at pH 5.0 . The optimized assay revealed limits of detection for the folate vitamers ranging between 2.0 and 5.6 pmol per assay (equivalent to 2.2 - 6.6 µg/ 100 g dry mass), recoveries ranging between 98 and 105 % and relative standard deviations in inter-assay precision ranging between 2 and 6 %. The assay was applied to quantitate folates in spinach, beans, cheeses, bread, wheat germs, and yeast . |
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Authors:
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Sabine Mönch; Michael Rychlik |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-11 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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